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Invitrogen
HYOU1 Monoclonal Antibody (6E3-2C3), PerCP
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FIGURE: 1 / 3
HYOU1 Antibody (MA5-45340) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GRP170 monoclonal antibody (Product # MA5-45340) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X. (A) DAPI (blue) nuclear stain... View More
Product Details
MA5-45340
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
6E3-2C3
Immunogen
Recombinant Full length GRP170 Protein
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45340 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 170kDa.
Target Information
HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 140 kDa Ca(2+)-binding protein; 150 kDa oxygen-regulated protein; 150-kDa oxygen regulated protein; 170 kDa glucose regulated protein GRP170; 170 kDa glucose-regulated protein; calcium binding protein 140; calcium binding protein, 140 kDa; CBP-140; DKFZp686N08236; epididymis secretory sperm binding protein; FLJ94899; FLJ97572; Grp 170; GRP-170; Heat shock protein family H member 4; HSP 12A; Hypoxia up-regulated protein 1; Orp 150; ORP-150; ORP150; oxygen regulated protein (150kD); unnamed protein product
Gene Aliases: AI415631; Cab140; CBP-140; GRP-170; GRP170; HSP12A; HSPH4; HYOU1; IMD59; ORP-150; ORP150
UniProt ID: (Human) Q9Y4L1, (Rat) Q63617, (Mouse) Q9JKR6
Entrez Gene ID: (Human) 10525, (Rat) 192235, (Mouse) 12282
response to ischemia
protein folding
ER to Golgi vesicle-mediated transport
response to stress
response to endoplasmic reticulum stress
negative regulation of apoptotic process
cellular response to hypoxia
regulation of cellular response to stress
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway
negative regulation of intrinsic apoptotic signaling pathway
response to hypoxia
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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