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Invitrogen

HO-1 Monoclonal Antibody (6B8-2F2), APC

View all (38) HO-1 antibodies

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Product Details

MA5-45346

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6B8-2F2

Immunogen

His-tagged Rat HO-1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931800

Product Specific Information

1 µg/mL of MA5-45346 was sufficient for detection of HO-1 in 10 µg of rat kidney lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 32kDa. Does not cross-react with HO-2.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HMOX1; HO-1; HSP32; P32 protein; unnamed protein product

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Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901

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Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368

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Function(s)
heme oxygenase (decyclizing) activity structural molecule activity protein binding oxidoreductase activity enzyme binding heme binding identical protein binding protein homodimerization activity metal ion binding phospholipase D activity signal transducer activity oxygenase
Process(es)
angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration regulation of transcription from RNA polymerase II promoter heme oxidation cellular iron ion homeostasis apoptotic process response to oxidative stress smooth muscle hyperplasia macroautophagy positive regulation of macroautophagy negative regulation of macroautophagy positive regulation of chemokine production erythrocyte homeostasis low-density lipoprotein particle clearance cellular response to heat response to nicotine intracellular signal transduction heme catabolic process heme metabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of angiogenesis positive regulation of angiogenesis positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation multicellular organismal iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to cisplatin positive regulation of cell migration involved in sprouting angiogenesis negative regulation of ferroptosis negative regulation of cytokine production involved in inflammatory response negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis epithelial cell apoptotic process positive regulation of epithelial cell apoptotic process response to hypoxia phospholipid metabolic process small GTPase mediated signal transduction regulation of blood pressure cell death negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process cellular response to nutrient negative regulation of mast cell cytokine production regulation of transcription from RNA polymerase II promoter in response to iron negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to hypoxia oxidation-reduction process
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