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Invitrogen
TRPC7 Monoclonal Antibody (N64A/36), PE
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FIGURE: 1 / 4
TRPC7 Antibody (MA5-45476) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TrpC7 monoclonal antibody (Product # MA5-45476) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 antibody (D) Composite.
Product Details
MA5-45476
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N64A/36
Immunogen
Synthetic peptide amino acids 845-862 of human TRPC7
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45476 was sufficient for detection of TrpC7 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.| Detects approximately 100kDa.
Target Information
TRPC7 is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC7 is probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC7 is also activated by diacylglycerol (DAG). Further, TRPC7 may also be activated by intracellular calcium store depletion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: capacitative calcium channel TRPC7; likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7; mTRP7; putative capacitative calcium channel; Short transient receptor potential channel 7; Transient receptor protein 7; TRP-7; TRP7 protein; TRPC7
Gene Aliases: TRP7; TRPC7; Trrp8
UniProt ID: (Human) Q9HCX4, (Mouse) Q9WVC5
Entrez Gene ID: (Human) 57113, (Rat) 282822, (Mouse) 26946
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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