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          • Primary Antibodies ›
          • GABRD Antibodies

          Invitrogen

          GABRD Monoclonal Antibody (N151/3), APC

          View all (20) GABRD antibodies

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          Cite GABRD Monoclonal Antibody (N151/3), APC

          Additional Information:
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          • Antibody Testing Data (3)
          GABRD Antibody in Immunocytochemistry (ICC/IF)
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          GABRD Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          GABRD Antibody (MA5-45481) in ICC/IF

          Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with GABA-A Receptor Delta monoclonal antibody (Product # MA5-45481) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor D... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          GABRD Antibody in Immunocytochemistry (ICC/IF)
          GABRD Antibody in Immunocytochemistry (ICC/IF)
          GABRD Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          GABRD Monoclonal Antibody (N151/3), APC

          Product Details

          MA5-45481

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (IHC)

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          Antibody Microarray (Ab Microarray)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          N151/3

          Immunogen

          Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta
          3D Epitope / Immunogen

          Conjugate

          APC APC APC
          • Unconjugated
          • FITC
          • PE 
          • PerCP 
          • Request custom conjugation

          Excitation/Emission Max

          651/660 nm View spectra spectra

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2931935

          Product Specific Information

          2 µg/mL of MA5-45481 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa.

          Target Information

          GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the gaba/benzodiazepine receptor and opening an integral chloride channel.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: GABA(A) receptor subunit delta; GABA(A) receptor, delta; GABA-A receptor; GABA-A receptor delta subunit; GABA-A receptor delta-subunit; GABA-A receptor, delta polypeptide; GABAA receptor delta subunit; GABAAR subunit delta; GABAARdelta; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor, delta; gamma-aminobutyric acid (GABA-A) receptor, subunit delta; gamma-aminobutyric acid A receptor, delta; gamma-aminobutyric acid type A receptor delta subunit; ion channel subunit; ligand-gated ion channel; MGC105467; MGC45284; unnamed protein product

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          Gene Aliases: EIG10; EJM7; GABAA-RD; GABAARdelta; GEFSP5

          View more View less

          UniProt ID: (Human) O14764, (Rat) P18506, (Mouse) P22933

          View more View less

          Entrez Gene ID: (Human) 2563, (Rat) 29689, (Mouse) 14403

          View more View less

          Function(s)
          transmembrane signaling receptor activity GABA-A receptor activity ion channel activity extracellular ligand-gated ion channel activity protein binding transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
          Process(es)
          ion transport gamma-aminobutyric acid signaling pathway cell-cell signaling chemical synaptic transmission ion transmembrane transport synaptic transmission, GABAergic regulation of postsynaptic membrane potential chloride transmembrane transport signal transduction extrasynaptic signaling via GABA
          It has to be done as per old AB suggested Products section.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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