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Invitrogen
GABRD Monoclonal Antibody (N151/3), FITC
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FIGURE: 1 / 3
GABRD Antibody (MA5-45482) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with GABA-A Receptor Delta monoclonal antibody (Product # MA5-45482) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor D... View More
Product Details
MA5-45482
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N151/3
Immunogen
Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
2 µg/mL of MA5-45482 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa.
Target Information
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the gaba/benzodiazepine receptor and opening an integral chloride channel.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor subunit delta; GABA(A) receptor, delta; GABA-A receptor delta-subunit; GABA-A receptor, delta polypeptide; GABAA receptor delta subunit; GABAAR subunit delta; GABAARdelta; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, delta; gamma-aminobutyric acid (GABA) A receptor, delta; gamma-aminobutyric acid (GABA-A) receptor, subunit delta; gamma-aminobutyric acid A receptor, delta; Gamma-aminobutyric acid receptor subunit delta; gamma-aminobutyric acid type A receptor delta subunit; ion channel subunit; MGC105467; MGC45284; unnamed protein product
Gene Aliases: AI853201; EIG10; EJM7; GABAA-RD; GABAARdelta; GABRD; GEFSP5
UniProt ID: (Human) O14764, (Mouse) P22933, (Rat) P18506
Entrez Gene ID: (Human) 2563, (Mouse) 14403, (Rat) 29689
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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