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Invitrogen
ATP7B Monoclonal Antibody (L62/29), PE
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FIGURE: 1 / 3
ATP7B Antibody (MA5-45525) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using NIH 3T3 (NIH 3T3). Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Copper Transporting ATPase 2 monoclonal antibody (Product # MA5-45525) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texa... View More
Product Details
MA5-45525
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
L62/29
Immunogen
Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45525 was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.|Detects approximately 160kDa in rat brain membrane preparations.
This antibody was formerly sold as clone S62-29.
Target Information
This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide (same as Wilson disease); Copper pump 2; copper transport protein; mutant protein; Copper-transporting ATPase 2; copper-transporting protein ATP7B; ORF; PINA gene, promoter; pineal night-specific ATPase; putative; RP11-327P2.1; RP11-327P2.3; toxic milk; unnamed protein product; Wilson disease-associated protein; Wilson disease-associated protein homolog; Wilson protein
Gene Aliases: Atp7a; ATP7B; Hts; PINA; PWD; tx; WC1; WD; WND
UniProt ID: (Human) P35670, (Mouse) Q64446
Entrez Gene ID: (Human) 540, (Rat) 24218, (Mouse) 11979
nucleotide binding
copper ion transmembrane transporter activity
copper ion binding
protein binding
ATP binding
ATPase activity
cation-transporting ATPase activity
copper-transporting ATPase activity
metal ion binding
P-type monovalent copper transporter activity
copper-exporting ATPase activity
zinc ion binding
hydrolase activity
primary active transporter
ion transport
cation transport
copper ion transport
cellular copper ion homeostasis
cellular zinc ion homeostasis
lactation
copper ion import
ion transmembrane transport
response to copper ion
sequestering of calcium ion
protein maturation
establishment of localization in cell
copper ion export
viral translational frameshifting
drug transmembrane export
circadian rhythm
metabolic process
response to zinc ion
intracellular copper ion transport
metal ion transport
copper ion transmembrane transport
response to cAMP
cation transmembrane transport
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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