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Invitrogen
ATP7B Monoclonal Antibody (L62/29), APC
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FIGURE: 1 / 3
ATP7B Antibody (MA5-45522) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using NIH 3T3 (NIH 3T3). Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Copper Transporting ATPase 2 monoclonal antibody (Product # MA5-45522) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45522
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
L62/29
Immunogen
Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45522 was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.|Detects approximately 160kDa in rat brain membrane preparations.
This antibody was formerly sold as clone S62-29.
Target Information
This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide (same as Wilson disease); copper pump 2; copper transport protein; mutant protein; copper-transporting protein ATP7B; ORF; PINA; PINA gene, promoter; pineal night-specific ATPase; putative; RP11-327P2.1; RP11-327P2.3; toxic milk; unnamed protein product; Wilson disease-associated protein; wilson disease-associated protein homolog; Wilson protein; Wilson; 's disease gene
Gene Aliases: Atp7a; Hts; PINA; PWD; tx; WC1; WD; WND
UniProt ID: (Human) P35670, (Mouse) Q64446
Entrez Gene ID: (Human) 540, (Mouse) 11979, (Rat) 24218
nucleotide binding
copper ion transmembrane transporter activity
copper ion binding
protein binding
ATP binding
ATPase activity
cation-transporting ATPase activity
metal ion binding
P-type monovalent copper transporter activity
zinc ion binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
copper-transporting ATPase activity
cation transport
copper ion transport
cellular copper ion homeostasis
copper ion import
ion transmembrane transport
response to copper ion
copper ion export
cellular detoxification of copper ion
drug transmembrane export
cellular zinc ion homeostasis
lactation
protein maturation
cellular response to copper ion
circadian rhythm
response to zinc ion
response to cAMP
cellular response to manganese ion
response to prolactin
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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