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Invitrogen
CLCN3 Monoclonal Antibody (N258/5), APC
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FIGURE: 1 / 3
CLCN3 Antibody (MA5-45554) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Clcn3 monoclonal antibody (Product # MA5-45554) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification:... View More
Product Details
MA5-45554
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N258/5
Immunogen
Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45554 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
This antibody was formerly sold as clone S258-5.
Target Information
Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chloride channel 3; Chloride channel protein 3; chloride channel, voltage-sensitive 3; Chloride transporter ClC-3; CIC3; ClC-3; CLC3; CLCN 3; clcn3e; DKFZp564I0463; H(+)/Cl(-) exchange transporter 3; protein kinase C-regulated chloride channel; unnamed protein product
Gene Aliases: ClC-3; CLC3; CLCN3; NEDHYBA; NEDSBA
UniProt ID: (Human) P51790, (Rat) P51792, (Mouse) P51791
Entrez Gene ID: (Human) 1182, (Rat) 84360, (Mouse) 12725
nucleotide binding
voltage-gated chloride channel activity
chloride channel activity
protein binding
ATP binding
chloride transmembrane transporter activity
antiporter activity
PDZ domain binding
chloride:proton antiporter activity
volume-sensitive chloride channel activity
ion channel activity
voltage-gated ion channel activity
protein heterodimerization activity
ion channel
ion transport
chloride transport
regulation of pH
phagocytosis, engulfment
adult locomotory behavior
synaptic transmission, glutamatergic
photoreceptor cell maintenance
negative regulation of cell volume
endosomal lumen acidification
synaptic transmission, GABAergic
transmembrane transport
neuron cellular homeostasis
synaptic vesicle lumen acidification
chloride transmembrane transport
positive regulation of reactive oxygen species biosynthetic process
ion transmembrane transport
regulation of ion transmembrane transport
regulation of anion transport
transport
regulation of anion transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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