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Invitrogen
Spectrin beta-4 Monoclonal Antibody (N393/2), PE
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FIGURE: 1 / 3
Spectrin beta-4 Antibody (MA5-45724) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with beta 4 Spectrin monoclonal antibody (Product # MA5-45724) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,... View More
Product Details
MA5-45724
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N393/2
Immunogen
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-45724 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately >200kDa. Does not cross react with other Beta-spectrins
This antibody was formerly sold as clone S393-2.
Target Information
Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart. Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 289 kDa isoform; 77 kDa isoform; beta SpIV; Beta-IV spectrin; beta-spectrin 4; cytoskeletal protein; alternatively spliced; EMBL BAB83243.1; lumbosacral neuroaxonal dystrophy; neuroaxonal dystrophy; quivering; spectrin beta 4; spectrin beta chain, brain 3; Spectrin beta chain, non-erythrocytic 4; Spectrin, non-erythroid beta chain 3; SPNB4
Gene Aliases: 1700022P15Rik; 5830426A08Rik; CMND; dyn; KIAA1642; lnd; NEDHND; nmf261; nmf379; QV; ROSA62; SpbIV; SPNB4; SPTBN3; SPTBN4
UniProt ID: (Human) Q9H254
Entrez Gene ID: (Human) 57731, (Mouse) 80297, (Rat) 308458
regulation of sodium ion transport
axonogenesis
neuromuscular junction development
sensory perception of sound
adult walking behavior
protein localization
fertilization
negative regulation of heart rate
vesicle-mediated transport
transmission of nerve impulse
central nervous system projection neuron axonogenesis
actin cytoskeleton organization
adult behavior
positive regulation of multicellular organism growth
clustering of voltage-gated sodium channels
neuromuscular process
actin filament capping
cardiac conduction
protein-containing complex assembly
protein localization to plasma membrane
reproductive process
regulation of peptidyl-serine phosphorylation
cellular protein localization
establishment of protein localization to plasma membrane
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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