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Invitrogen
Spectrin beta-4 Monoclonal Antibody (N393/2), FITC
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FIGURE: 1 / 3
Spectrin beta-4 Antibody (MA5-45722) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with beta 4 Spectrin monoclonal antibody (Product # MA5-45722) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45722
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N393/2
Immunogen
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-45722 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately >200kDa. Does not cross react with other Beta-spectrins
This antibody was formerly sold as clone S393-2.
Target Information
Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart. Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 289 kDa isoform; 77 kDa isoform; beta SpIV; beta-IV spectrin; beta-spectrin 4; cytoskeletal protein; cytoskeletal protein; alternatively spliced; EMBL BAB83243.1; lumbosacral neuroaxonal dystrophy; neuroaxonal dystrophy; quivering; spectrin beta 4; spectrin beta chain, brain 3; spectrin, non-erythroid beta chain 3; SPNB4; unnamed protein product
Gene Aliases: 1700022P15Rik; 5830426A08Rik; CMND; dyn; lnd; NEDHND; nmf261; nmf379; QV; ROSA62; SpbIV; SPNB4; SPTBN3
UniProt ID: (Human) Q9H254
Entrez Gene ID: (Human) 57731, (Rat) 308458, (Mouse) 80297
regulation of sodium ion transport
axonogenesis
sensory perception of sound
negative regulation of heart rate
vesicle-mediated transport
transmission of nerve impulse
central nervous system projection neuron axonogenesis
actin cytoskeleton organization
clustering of voltage-gated sodium channels
cardiac conduction
protein localization to plasma membrane
neuromuscular junction development
adult walking behavior
protein localization
biological_process
fertilization
adult behavior
regulation of peptidyl-serine phosphorylation
positive regulation of multicellular organism growth
neuromuscular process
protein-containing complex assembly
actin filament capping
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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