Immunohistochemistry analysis using Kidney. Samples were incubated with Malondialdehyde monoclonal antibody (Product # MA5-45805) at 1:100 for Overnight at 4C, then 30 min at 37C, followed by Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain used was DAPI for 3 min at RT. Magnification: 10X.

Malondialdehyde Antibody in Western Blot (WB)

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Product Details

MA5-45805

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

1:100

Immunocytochemistry (ICC/IF)

1:50

ELISA (ELISA)

1:1,000

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

11E3

Immunogen

Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Protein G

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2932259

Product Specific Information

A 1:1,000 dilution of MA5-45805 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.

Target Information

Malondialdehyde is an organic compound with the formula CH2(CHO)2, and a byproduct of lipid metabolism in the body. Malondialdehyde, a highly reactive compound, is one of the many reactive electrophile species that cause toxic stress in cells and form covalent protein adducts, called advanced lipoxidation end products (ALE). This compound also forms mutagenic DNA adducts when it reacts with deoxyadenosine and deoxyguanosine in DNA. Malondialdehyde is also found in many foods and is present in high levels in rancid foods.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-Propanedial; Malonaldehyde; Malondialdehyde; MDA

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Malondialdehyde Monoclonal Antibody (11E3), APC

Malondialdehyde Antibody (MA5-45805) in IHC