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Invitrogen

nNOS Monoclonal Antibody (J23), FITC

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Product Details

MA5-45958

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

J23

Immunogen

Partial Human Recombinant NOS1 Protein

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932412

Product Specific Information

A 1:1,000 dilution of MA5-45958 was sufficient for detection of NOS1/Nnos in 7.5 µg of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Detects approximately 160.97 kDa.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; unnamed protein product

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Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1

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UniProt ID: (Human) P29475, (Rat) P29476, (Mouse) Q9Z0J4

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Entrez Gene ID: (Human) 4842, (Rat) 24598, (Mouse) 18125

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Function(s)
nitric-oxide synthase activity calcium channel regulator activity protein binding calmodulin binding FMN binding oxidoreductase activity sodium channel regulator activity heme binding tetrahydrobiopterin binding arginine binding peptidyl-cysteine S-nitrosylase activity ion channel binding cadmium ion binding metal ion binding calcium-dependent protein binding flavin adenine dinucleotide binding NADP binding scaffold protein binding NADPH-hemoprotein reductase activity iron ion binding amino acid binding enzyme binding protein homodimerization activity ATPase binding oxidoreductase
Process(es)
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process potassium ion transport calcium ion transport striated muscle contraction cell communication nitric oxide mediated signal transduction myoblast fusion blood circulation response to heat response to hormone negative regulation of calcium ion transport into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of metal ion transport signaling response to lipopolysaccharide multicellular organismal response to stress xenobiotic catabolic process vasodilation negative regulation of potassium ion transport negative regulation of ion transport positive regulation of neuron apoptotic process cell redox homeostasis negative regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis negative regulation of serotonin uptake establishment of localization in cell negative regulation of calcium ion transport regulation of cardiac muscle contraction regulation of postsynaptic membrane potential regulation of biological quality cellular response to growth factor stimulus positive regulation of the force of heart contraction synaptic signaling by nitric oxide positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of blood circulation positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential muscle contraction female pregnancy aging regulation of heart contraction negative regulation of cell proliferation response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to nutrient levels negative regulation of peptidyl-serine phosphorylation response to vitamin E positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process response to peptide hormone response to estrogen response to ethanol negative regulation of heart contraction negative regulation of vasoconstriction positive regulation of vasodilation negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration regulation of sensory perception of pain oxidation-reduction process cellular response to mechanical stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death
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