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Invitrogen

nNOS Monoclonal Antibody (J23), APC

View all (32) nNOS antibodies

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Product Details

MA5-45957

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

J23

Immunogen

Partial Human Recombinant NOS1 Protein

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932411

Product Specific Information

A 1:1,000 dilution of MA5-45957 was sufficient for detection of NOS1/Nnos in 7.5 µg of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Detects approximately 160.97 kDa.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bNOS1; Brain NOS; constitutive NOS; N-NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; nNOS mu; NOS Type 1; NOS type I; peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; unnamed protein product

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Gene Aliases: 2310005C01Rik; bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I

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UniProt ID: (Human) P29475, (Mouse) Q9Z0J4, (Rat) P29476

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Entrez Gene ID: (Human) 4842, (Mouse) 18125, (Rat) 24598

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Function(s)
nitric-oxide synthase activity calcium channel regulator activity protein binding calmodulin binding FMN binding oxidoreductase activity sodium channel regulator activity heme binding tetrahydrobiopterin binding arginine binding peptidyl-cysteine S-nitrosylase activity ion channel binding cadmium ion binding calcium-dependent protein binding flavin adenine dinucleotide binding NADP binding scaffold protein binding zinc ion binding enzyme binding identical protein binding ATPase binding phosphoprotein binding RNA polymerase II sequence-specific DNA binding transcription factor binding NADPH binding
Process(es)
response to hypoxia regulation of the force of heart contraction regulation of sodium ion transport system process arginine catabolic process nitric oxide biosynthetic process striated muscle contraction cell communication negative regulation of cell proliferation response to heat response to hormone peptidyl-cysteine S-nitrosylation signaling response to lipopolysaccharide multicellular organismal response to stress negative regulation of iron ion transmembrane transport nitric oxide-cGMP-mediated signaling pathway xenobiotic catabolic process vasodilation negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to estrogen establishment of protein localization positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of blood pressure positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine regulation of neurogenesis negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of postsynaptic membrane potential negative regulation of hepatic stellate cell contraction cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epinephrine stimulus positive regulation of adrenergic receptor signaling pathway positive regulation of the force of heart contraction synaptic signaling by nitric oxide positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of long-term synaptic potentiation positive regulation of sodium ion transmembrane transport myoblast fusion blood circulation negative regulation of calcium ion transport into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of metal ion transport negative regulation of ion transport cell redox homeostasis regulation of cardiac muscle contraction regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of blood circulation positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential female pregnancy response to mechanical stimulus response to lead ion response to activity response to nutrient levels response to vitamin E response to vitamin B3 response to nicotine response to decreased oxygen levels regulation of apoptotic process response to ethanol nitric oxide metabolic process response to nitric oxide
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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