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Invitrogen
MAFG Polyclonal Antibody
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FIGURE: 1 / 1
MAFG Antibody (PA5-90907) in WB
Western blot analysis of MAFG in extracts of various cell lines. Samples were incubated with MAFG Polyclonal antibody (Product # PA5-90907) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-90907
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human MAFG (NP_0023501)
Conjugate
Form
Liquid
Concentration
3.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV ITIVKSKTDA RS; Positive Samples: U-87MG, A-549, HT-29, 293T; Cellular Location: Nucleus
Target Information
Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: avian musculoaponeurotic fibrosarcoma virus (v-maf) AS42 oncogene, protein G; basic leucine zipper transcription factor MafG; hMAF; MGC13090; MGC20149; Transcription factor MafG; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G; V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
Gene Aliases: AA545192; C630022N07Rik; hMAF; MAFG
UniProt ID: (Human) O15525, (Mouse) O54790
Entrez Gene ID: (Human) 4097, (Mouse) 17134
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
protein heterodimerization activity
DNA binding
basic leucine zipper transcription factor
in utero embryonic development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
blood coagulation
adult behavior
regulation of cellular pH
regulation of cell proliferation
regulation of epidermal cell differentiation
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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