Proteintech

MAP2 Polyclonal Antibody

Product Image Gallery

MAP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of MAP2 (17490-1-AP, 1:250 dilution) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (RED MAP2; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

MAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse cerebellum tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MAP2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA ) fixed mouse brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MAP2 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

MAP2 Antibody in Immunoprecipitation (IP) — IP result of anti-MAP2 (IP:17490-1-AP, 3ug; Detection:17490-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:400

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 24 publications 24 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MAP2 Fusion Protein Ag11580

Conjugate

Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can recognize both high MW and low MW isoforms of MAP2.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.