View of mixed neuron/glial cultures stained with CPCA-MAP2 (red). The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.
|Tested species reactivity||Human, Mouse, Rat, Bovine|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified MAP2 isolated from bovine brain|
|Purification||Ammonium sulfate precipitation|
|Contains||5mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000-1:5000|
|Western Blot (WB)||1:10,000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot bands can be seen at approximately 280 kDa, representing MAP2a and MAP2b.
Bands may also be seen at approximately 70 kDa, representing MAP2c.
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.