Immunofluorescent analysis of MAP2 in the differentiated neurons from H9 ESC-derived NSCs . 2 weeks after differentiation, cells were fixed, permeabilized and stained with a MAP2 rabbit polyclonal antibody (Product # PA5-17646) at 1:100 dilution (green) and a HuC/HuD mouse monoclonal antibody (Product # A21271), at a concentration of 5 ug/ml (red) in blocking buffer for at least 1 hour at room temperature, and then incubated with goat anti-rabbit IgG secondary antibody, Alexa Fluor Plus 488 conjugate (Product # A32731, green) and a donkey anti-mouse IgG secondary antibody, Alexa Fluor 594 conjugate (Product # A21203, red) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249).
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to carboxy-terminal residues of human MAP2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
PA5-17646 was successfully used to detect MAP2 in neurons differentiated from H9 ESC derived NSCs.
This antibody is not cross-reactive with tau.
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
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