(A) HEK293T cells were transiently transfected with an expression vector for HA-tagged MeCP2. Whole cell extracts were immunoprecipitated with an antibody against the HA-tag (positive control; 2 µg) (lane 1), with the anti-MeCP2 antibody (2 µg) (Cat. no. 49-1029) (lane 2), and with beads only (lane 3). The presence of HA-MeCP2 in the immunoprecipitates was demonstrated by western blot analysis with an antibody directed against HA. (B) Nuclear extracts from untransfected HeLa cells were immunoprecipitated with anti-MeCP2 antibody (Cat. no. 49-1029) (2 µg) and beads only (lanes 5 and 6). The presence of MeCP in the immunoprecipitates and in the unprecipitated nuclear extracts (lane 4) was demonstrated by western blot analysis with the MeCP2 antibody (0.4 µg⁄ml).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the C-term of human MECP2.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||8.5 ug|
|ELISA (ELISA)||1:500 to 1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain. Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.