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Invitrogen
MECP2 Polyclonal Antibody
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FIGURE: 1 / 14
MECP2 Antibody (PA1-887) in ICC/IF
Immunofluorescence analysis of Mecp2 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MECP2 Polyclonal Antibody (Product # PA1-887) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000), f... View More
Product Details
PA1-887
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Fruit fly,
Human,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-887 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat tissues and cells.
PA1-887 has been successfully used in Western blot, IHC-P, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.
PA1-887 immunizing peptide corresponds to amino acid residues 1-15 from mouse MeCP2. This sequence is completely conserved in human MeCP2. PA1-887 immunizing peptide (Cat. # PEP-120) is available for use in neutralization and control experiments.
Target Information
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686A24160; MeCp-2 protein; methyl CpG binding protein 2; Methyl-CpG-binding protein 2; testis tissue sperm-binding protein Li 41a
Gene Aliases: 1500041B07Rik; AUTSX3; D630021H01Rik; Mbd5; MECP2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP10
UniProt ID: (Human) P51608, (Mouse) Q9Z2D6, (Rat) Q00566
Entrez Gene ID: (Human) 4204, (Mouse) 17257, (Rat) 29386
four-way junction DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
mRNA binding
protein binding
transcription factor binding
methyl-CpG binding
double-stranded methylated DNA binding
protein domain specific binding
chromatin DNA binding
siRNA binding
histone deacetylase binding
poly(A) RNA binding
unmethylated CpG binding
protein N-terminus binding
chromatin binding
enzyme binding
DNA metabolism protein
negative regulation of transcription from RNA polymerase II promoter
behavioral fear response
response to hypoxia
startle response
neurological system process involved in regulation of systemic arterial blood pressure
regulation of respiratory gaseous exchange by neurological system process
inositol metabolic process
mitochondrial electron transport, ubiquinol to cytochrome c
chromatin silencing
regulation of gene expression by genetic imprinting
transcription, DNA-templated
glutamine metabolic process
cellular biogenic amine metabolic process
synapse assembly
heart development
respiratory gaseous exchange
long-term memory
locomotory behavior
protein localization
glucocorticoid metabolic process
positive regulation of cell proliferation
adult locomotory behavior
visual learning
response to radiation
pathogenesis
post-embryonic development
response to ionizing radiation
response to lead ion
dendrite development
histone methylation
histone acetylation
proprioception
sensory perception of pain
cerebellum development
ventricular system development
negative regulation of histone methylation
cardiolipin metabolic process
response to estradiol
negative regulation of histone acetylation
social behavior
neuron maturation
negative regulation of neuron apoptotic process
regulation of DNA methylation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
phosphatidylcholine metabolic process
negative regulation of astrocyte differentiation
catecholamine secretion
positive regulation of synapse assembly
excitatory postsynaptic potential
long-term synaptic potentiation
regulation of transcription, DNA-templated
chemical synaptic transmission
brain development
learning
memory
embryo development
regulation of gene expression
neuron differentiation
neuron projection development
multicellular organismal response to stress
regulation of gene expression, epigenetic
regulation of synaptic plasticity
neuromuscular process controlling posture
neuromuscular process
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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