Immunofluorescent analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Methyl CpG Binding Protein 2 polyclonal antibody (Product # PA1-887) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:2000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-887 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat tissues and cells.
PA1-887 has been successfully used in Western blot, IHC-P, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.
PA1-887 immunizing peptide corresponds to amino acid residues 1-15 from mouse MeCP2. This sequence is completely conserved in human MeCP2. PA1-887 immunizing peptide (Cat. # PEP-120) is available for use in neutralization and control experiments.
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the Sin3 and histone deactylase protein families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Effects of Maternal Separation on Adult Methamphetamine Self-Administration, Extinction, Reinstatement, and MeCP2 Immunoreactivity in the Nucleus Accumbens.
PA1-887 was used in immunohistochemistry to study adult methamphetamine self-administration, methamphetamine-seeking behaviour and nucleus accumbens MeCP2 levels following maternal separation in rats
|Lewis CR,Staudinger K,Scheck L,Olive MF||Frontiers in psychiatry (4:null)||2013|
Multiple pathways regulate MeCP2 expression in normal brain development and exhibit defects in autism-spectrum disorders.
PA1-887 was used in immunohistochemistry to study the regulatory mechanisms of the MeCP2 expression during brain development
|Samaco RC,Nagarajan RP,Braunschweig D,LaSalle JM||Human molecular genetics (13:629)||2004|
Quantitative localization of heterogeneous methyl-CpG-binding protein 2 (MeCP2) expression phenotypes in normal and Rett syndrome brain by laser scanning cytometry.
PA1-887 was used in immunohistochemistry to examine the expression profile of methyl-CpG-binding protein 2 in normal and Rett syndrome brains.
|LaSalle JM,Goldstine J,Balmer D,Greco CM||Human molecular genetics (10:1729)||2001|
|Non-human primate||Not Cited||
The first missense mutation causing Rett syndrome specifically affecting the MeCP2_e1 isoform.
PA1-887 was used in immunocytochemistry to investigate a missense mutation in MeCP2_e1 isoform in Rett syndrome
|Fichou Y,Nectoux J,Bahi-Buisson N,Rosas-Vargas H,Girard B,Chelly J,Bienvenu T||Neurogenetics (10:127)||2009|
RNAi-induced down-regulation of Mecp2 expression in the rat brain.
PA1-887 was used in western blot to develop a rat model with lowered Mecp2 expression in the brain
|Jin J,Bao X,Wang H,Pan H,Zhang Y,Wu X||International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience (26:457)||2008|
A WW domain binding region in methyl-CpG-binding protein MeCP2: impact on Rett syndrome.
PA1-887 was used in western blot to study the domain structure of methyl-CpG-binding protein MeCP2 and its involvement in Rett syndrome
|Buschdorf JP,Strätling WH||Journal of molecular medicine (Berlin, Germany) (82:135)||2004|