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Invitrogen
CD19 Monoclonal Antibody (SJ25-C1), APC-Alexa Fluor™ 750
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Product Details
MHCD1927
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
SJ25-C1
Immunogen
Nalm 1 Human cell line
Conjugate
Excitation/Emission Max
756/785 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS with 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The allophycocyanin (APC)-Alexa Fluor™ 750 dye tandem conjugate allows for multicolor measurements using excitation by lasers that emit from 633 to 650 nm.
Target Information
CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.
Analyte Specific Reagent
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; CD19 differentiation antigen; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12; unnamed protein product
Gene Aliases: B4; CD19; CVID3
UniProt ID: (Human) P15391
Entrez Gene ID: (Human) 930
B-1 B cell differentiation
adaptive immune response
B cell proliferation involved in immune response
immune system process
immunoglobulin mediated immune response
B cell mediated immunity
B cell proliferation
antigen receptor-mediated signaling pathway
B cell receptor signaling pathway
regulation of B cell receptor signaling pathway
regulation of B cell activation
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
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