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Invitrogen
CD19 Monoclonal Antibody (SJ25-C1), Pacific Blue™
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FIGURE: 1 / 4
CD19 Antibody (MHCD1928) in Flow
The Journal of clinical investigation 2024 -
Loss of CD19 expression in CD19 CAR-T-treated pediatric patients with ALL corresponds to a loss of CD22 expression. ( A and B ) Reduced CD19 and CD22 expression on resistant cells seen by FACS analysis. Gating was based on the isotype control. ( C and D ) CD19 and CD22 antigen expression was observed in a cohort of cells from pediatric patients with ALL ( n = 11) before treatment and after relapse following... View More
Product Details
MHCD1928
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
SJ25-C1
Immunogen
Nalm 1 Human cell line
Conjugate
Excitation/Emission Max
404/455 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS with 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
Target Information
CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.
Analyte Specific Reagent
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; CD19 differentiation antigen; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12; unnamed protein product
Gene Aliases: B4; CD19; CVID3
UniProt ID: (Human) P15391
Entrez Gene ID: (Human) 930
B-1 B cell differentiation
adaptive immune response
B cell proliferation involved in immune response
immune system process
immunoglobulin mediated immune response
B cell mediated immunity
B cell proliferation
antigen receptor-mediated signaling pathway
B cell receptor signaling pathway
regulation of B cell receptor signaling pathway
regulation of B cell activation
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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