Proteintech

MLH1 Polyclonal Antibody

MLH1 Antibody in Immunocytochemistry (ICC/IF)

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MLH1 Antibody (11697-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11697-1-AP (MLH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MLH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MLH1 Antibody in Immunoprecipitation (IP)

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Product Details

11697-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MLH1 Fusion Protein Ag2319 (407-757 aa encoded by BC006850)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to MLH1.

Immunogen sequence: QPQAIVTED KTDISSGRAR QQDEEMLELP APAEVAAKNQ SLEGDTTKGT SEMSEKRGPT SSNPRKRHRE DSDVEMVEDD SRKEMTAACT PRRRIINLTS VLSLQEEINE QGHEVLREML HNHSFVGCVN PQWALAQHQT KLYLLNTTKL SEELFYQILI YDFANFGVLR LSEPAPLFDL AMLALDSPES GWTEEDGPKE GLAEYIVEFL KKKAEMLADY FSLEIDEEGN LIGLPLLIDN YVPPLEGLPI FILRLATEVN WDEEKECFES LSKECAMFYS IRKQYISEES TLSGQQSEVP GSIPNSWKWT VEHIVYKALR SHILPPKHFT EDGNILQLAN LPDLYKVFER C (407-757 aa encoded by BC006850)

Target Information

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inducible mismatch repair streamlines forward genetic approaches to target identification of cytotoxic small molecules.

bioRxiv : the preprint server for biology

Nguyen TP,Fang M,Kim J,Wang B,Lin E,Khivansara V,Barrows N,Rivera-Cancel G,Goralski M,Cervantes CL,Xie S,Peterson JM,Povedano JM,Antczak MI,Posner BA,McFadden DG,Ready JM,De Brabander JK,Nijhawan D

Tue Feb 21 00:00:00 UTC 2023

Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice.

Progress in neurobiology

Bai D,Zhu L,Jia Q,Duan X,Chen L,Wang X,Hou J,Jiang G,Yang S,Li S,Li XJ,Yin P

Tue Aug 01 00:00:00 UTC 2023

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Journal of hematology & oncology

Xu N,Yao Z,Shang G,Ye D,Wang H,Zhang H,Qu Y,Xu F,Wang Y,Qin Z,Zhu J,Zhang F,Feng J,Tian S,Liu Y,Zhao J,Hou J,Guo J,Hou Y,Ding C

Fri Jun 03 00:00:00 UTC 2022

The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.

Cell reports

Zhang XZ,Wei LL,Jin HJ,Zhang XH,Chen SR

Tue Jul 05 00:00:00 UTC 2022

Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.

Development (Cambridge, England)

Zhang XZ,Wei LL,Zhang XH,Jin HJ,Chen SR

Wed Jun 15 00:00:00 UTC 2022

Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.

Cancer immunology research

Wu Q,Huang Q,Jiang Y,Sun F,Liang B,Wang J,Hu X,Sun M,Ma Z,Shi Y,Liang Y,Tan Y,Zeng D,Yao F,Xu X,Yao Z,Li S,Rong X,Huang N,Sun L,Liao W,Shi M

Tue Feb 01 00:00:00 UTC 2022

Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.

Frontiers in oncology

Ran X,Hu T,Li Z

Sat Jul 16 00:00:00 UTC 2022

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Nucleic acids research

Chen L,Wang WJ,Liu Q,Wu YK,Wu YW,Jiang Y,Liao XQ,Huang F,Li Y,Shen L,Yu C,Zhang SY,Yan LY,Qiao J,Sha QQ,Fan HY

Fri Oct 28 00:00:00 UTC 2022

C. tropicalis promotes chemotherapy resistance in colon cancer through increasing lactate production to regulate the mismatch repair system.

International journal of biological sciences

Qu J,Sun Z,Peng C,Li D,Yan W,Xu Z,Hou Y,Shen S,Chen P,Wang T

Thu Mar 17 00:00:00 UTC 2022

Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.

Human molecular genetics

Bai D,Yin P,Zhang Y,Sun F,Chen L,Lin L,Yan S,Li S,Li XJ

Wed Jul 28 00:00:00 UTC 2021

Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.

Nucleic acids research

Li J,Liu F,Lv Y,Sun K,Zhao Y,Reilly J,Zhang Y,Tu J,Yu S,Liu X,Qin Y,Huang Y,Gao P,Jia D,Chen X,Han Y,Shu X,Luo D,Tang Z,Liu M

Fri Feb 26 00:00:00 UTC 2021

Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.

Journal of surgical oncology

Zhu J,Ke G,Bi R,Wu X

Tue Sep 01 00:00:00 UTC 2020

Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.

Journal of experimental & clinical cancer research : CR

Hu Z,Long T,Ma Y,Zhu J,Gao L,Zhong Y,Wang X,Wang X,Li Z

Tue May 05 00:00:00 UTC 2020

Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.

Journal of experimental & clinical cancer research : CR

Hu Z,Long T,Ma Y,Zhu J,Gao L,Zhong Y,Wang X,Wang X,Li Z

Tue May 05 00:00:00 UTC 2020

Downregulation of GLYR1 contributes to microsatellite instability colorectal cancer by targeting p21 via the p38MAPK and PI3K/AKT pathways.

Journal of experimental & clinical cancer research : CR

Hu Z,Long T,Ma Y,Zhu J,Gao L,Zhong Y,Wang X,Wang X,Li Z

Tue May 05 00:00:00 UTC 2020

Combinatorial approach of in silico and in vitro evaluation of MLH1 variant associated with Lynch syndrome like metastatic colorectal cancer.

Bioscience reports

Saleem K,Zaib T,Ji W,Zhang C,Qin Q,Wang Y,Xu L,Yu H,Zhu S,Dong K,Si S,Jia X,Wu J,Fu S,Sun W

Fri Jun 26 00:00:00 UTC 2020

Combinatorial approach of in silico and in vitro evaluation of MLH1 variant associated with Lynch syndrome like metastatic colorectal cancer.

Bioscience reports

Saleem K,Zaib T,Ji W,Zhang C,Qin Q,Wang Y,Xu L,Yu H,Zhu S,Dong K,Si S,Jia X,Wu J,Fu S,Sun W

Fri Jun 26 00:00:00 UTC 2020

The testis-specific gene 1700102P08Rik is essential for male fertility.

Molecular reproduction and development

Wu XL,Yun DM,Gao S,Liang AJ,Duan ZZ,Wang HS,Wang GS,Sun F

Sat Feb 01 00:00:00 UTC 2020

Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.

Surgery

Liang D,Shi S,Liang C,Meng Q,Zhang B,Ni Q,Xu J,Yu X

Tue May 01 00:00:00 UTC 2018

Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.

Surgery

Liang D,Shi S,Liang C,Meng Q,Zhang B,Ni Q,Xu J,Yu X

Tue May 01 00:00:00 UTC 2018

Clinical significance of chromodomain helicase/ATPase DNA binding protein 1-like and human mutL homolog 1 gene expression in cholangiocarcinoma.

Oncology letters

Hua J,Li S,Huang C

Sat Sep 01 00:00:00 UTC 2018

Clinical significance of chromodomain helicase/ATPase DNA binding protein 1-like and human mutL homolog 1 gene expression in cholangiocarcinoma.

Oncology letters

Hua J,Li S,Huang C

Sat Sep 01 00:00:00 UTC 2018

Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.

Nucleic acids research

Yuan Y,Zhao Y,Chen L,Wu J,Chen G,Li S,Zou J,Chen R,Wang J,Jiang F,Tang Z

Wed Sep 06 00:00:00 UTC 2017

Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.

Nucleic acids research

Yuan Y,Zhao Y,Chen L,Wu J,Chen G,Li S,Zou J,Chen R,Wang J,Jiang F,Tang Z

Wed Sep 06 00:00:00 UTC 2017

A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.

Medicine

Yuan LQ,Wang JH,Zhu K,Yang M,Gu WZ,Lai C,Li HM,Shu Q,Chen X

Fri Dec 01 00:00:00 UTC 2017

A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.

Medicine

Yuan LQ,Wang JH,Zhu K,Yang M,Gu WZ,Lai C,Li HM,Shu Q,Chen X

Fri Dec 01 00:00:00 UTC 2017

Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.

Experimental & molecular medicine

He YQ,Sheng JQ,Ling XL,Fu L,Jin P,Yen L,Rao J

Mon Dec 31 00:00:00 UTC 2012

Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.

Experimental & molecular medicine

He YQ,Sheng JQ,Ling XL,Fu L,Jin P,Yen L,Rao J

Mon Dec 31 00:00:00 UTC 2012

Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.

Cancer prevention research (Philadelphia, Pa.)

Jin P,Lu XJ,Sheng JQ,Fu L,Meng XM,Wang X,Shi TP,Li SR,Rao J

Sun Aug 01 00:00:00 UTC 2010

Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.

Cancer prevention research (Philadelphia, Pa.)

Jin P,Lu XJ,Sheng JQ,Fu L,Meng XM,Wang X,Shi TP,Li SR,Rao J

Sun Aug 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; MGC5172; mismatch repair protein 1; MLH-1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1

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Gene Aliases: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MLH1

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UniProt ID: (Human) P40692, (Rat) P97679, (Mouse) Q9JK91

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Entrez Gene ID: (Human) 4292, (Rat) 81685, (Mouse) 17350

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Function(s)

chromatin binding single-stranded DNA binding protein binding ATP binding ATPase activity guanine/thymine mispair binding MutSalpha complex binding molecular_function mismatched DNA binding structure-specific DNA binding DNA metabolism protein

Process(es)

nuclear-transcribed mRNA poly(A) tail shortening resolution of meiotic recombination intermediates mismatch repair double-strand break repair via nonhomologous end joining male meiosis chromosome segregation synapsis spermatogenesis intrinsic apoptotic signaling pathway in response to DNA damage female meiosis chromosome segregation somatic hypermutation of immunoglobulin genes meiotic metaphase I plate congression meiotic telomere clustering isotype switching negative regulation of mitotic recombination oogenesis meiotic spindle midzone assembly response to hypoxia somatic recombination of immunoglobulin genes involved in immune response DNA repair cellular response to DNA damage stimulus meiotic nuclear division reciprocal meiotic recombination male meiosis response to toxic substance somatic recombination of immunoglobulin gene segments response to drug meiotic chromosome segregation homologous chromosome segregation cellular response to organic cyclic compound cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MLH1 Polyclonal Antibody

MLH1 Antibody (11697-1-AP) in ICC/IF

Product Details

11697-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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