Proteintech

MLH1 Polyclonal Antibody

Product Image Gallery

MLH1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11697-1-AP (MLH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MLH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 11697-1-AP (MLH1 antibody) at dilution of 1:50 (under 40x lens).

MLH1 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MLH1 Antibody in Immunoprecipitation (IP) — IP result of anti-MLH1 (IP:11697-1-AP, 3ug; Detection:11697-1-AP 1:500) with HeLa cells lysate 3200ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:1000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MLH1 Fusion Protein Ag2319

Conjugate

Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to MLH1.

Target Information

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; MGC5172; mismatch repair protein 1; MLH-1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1

Gene Aliases: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MLH1

UniProt ID: (Human) P40692, (Rat) P97679, (Mouse) Q9JK91

Entrez Gene ID: (Human) 4292, (Rat) 81685, (Mouse) 17350

Molecular Function: DNA binding protein nucleic acid binding