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Invitrogen
MLH1 Recombinant Rabbit Monoclonal Antibody (R6E5)
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FIGURE: 1 / 1
MLH1 Antibody (MA572228) in WB
Western blot analysis of MLH1 in A-549, Colon, HeLa, Jurkat, SH-SY5Y, and Thymus lysates (25 µg/lane). Samples were incubated with MLH1 Recombinant Monoclonal (Product # MA572228) at 1:1,000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Product Details
MA572228
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
R6E5
Immunogen
KLH conjugated synthetic peptide derived from human MLH1.
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Protein A
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; DNA mismatch repair protein MutL homolog; human homolog of E. coli mutL gene product, Swiss-Prot Accession Number P23367; MGC5172; MLH-1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1; unnamed protein product
Gene Aliases: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; FCC2; hMLH1; HNPCC; HNPCC2; LYNCH2; MLH-1; MLH1; MMRCS1
UniProt ID: (Human) P40692, (Mouse) Q9JK91
Entrez Gene ID: (Human) 4292, (Mouse) 17350
nucleotide binding
chromatin binding
single-stranded DNA binding
protein binding
ATP binding
enzyme activator activity
ATPase activity
enzyme binding
mismatched DNA binding
guanine/thymine mispair binding
MutSalpha complex binding
ATP-dependent DNA damage sensor activity
structure-specific DNA binding
DNA metabolism protein
nuclear-transcribed mRNA poly(A) tail shortening
resolution of meiotic recombination intermediates
somatic recombination of immunoglobulin genes involved in immune response
DNA repair
mismatch repair
double-strand break repair via nonhomologous end joining
response to stress
cellular response to DNA damage stimulus
male meiosis chromosome segregation
synapsis
reciprocal meiotic recombination
male meiosis
spermatogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
response to bacterium
female meiosis chromosome segregation
somatic hypermutation of immunoglobulin genes
somatic recombination of immunoglobulin gene segments
meiotic metaphase I plate congression
meiotic chromosome segregation
meiotic telomere clustering
homologous chromosome segregation
isotype switching
negative regulation of mitotic recombination
positive regulation of isotype switching to IgA isotypes
positive regulation of isotype switching to IgG isotypes
oogenesis
meiotic spindle midzone assembly
meiotic cell cycle
cell cycle
meiotic nuclear division
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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