|Western Blot (WB)||2-3 ug/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 375–386 of human Muc 18|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with non-human primate based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
MUC18, also known as CD146 is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. The protein is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, MUC18 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; MCAM; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; S-endo 1 endothelial-associated antigen
Gene Aliases: CD146; MCAM; MUC18
UniProt ID: (Human) P43121
Entrez Gene ID: (Human) 4162
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