Western blot analysis of MCAM in whole cell extracts from Du-145 (lane 1), PC-3 (lane 2), and Sk-Mel-37 (lane 3) using a MCAM recombinant rabbit monoclonal antibody (Product # 701199) at a dilution of 2ug/ml. Samples were detected using chemiluminescence (ECL). Results show a band at ~110kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 375–386 of human Muc 18|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with non-human primate based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
MUC18, also known as CD146 is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. The protein is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, MUC18 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.