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Invitrogen
NLK Polyclonal Antibody
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FIGURE: 1 / 3
NLK Antibody (PA5-102708) in ICC/IF
Immunofluorescent analysis of NLK in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NLK polyclonal antibody (Product # PA5-102708) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102708
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NLK(Accession Q9UBE8), corresponding to amino acid residues H67-A117.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NLK.
Target Information
NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nemo-like kinase; NLK; Protein LAK1; serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK; similar to mouse nemo-like kinase (Nlk); unnamed protein product
Gene Aliases: AI194375; LAK1; NLK; RGD1561602
UniProt ID: (Human) Q9UBE8, (Mouse) O54949, (Rat) D3ZSZ3
Entrez Gene ID: (Human) 51701, (Mouse) 18099, (Rat) 497961
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
kinase activity
transferase activity
ubiquitin protein ligase binding
SH2 domain binding
metal ion binding
protein serine kinase activity
DNA-binding transcription factor binding
transcription factor binding
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
regulation of transcription, DNA-templated
protein phosphorylation
signal transduction
transforming growth factor beta receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
regulation of signal transduction
Wnt signaling pathway
peptidyl-threonine phosphorylation
negative regulation of Wnt signaling pathway
cellular response to nutrient levels
cellular response to stress
intracellular signal transduction
TORC1 signaling
protein stabilization
protein localization to lysosome
cellular response to osmotic stress
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of STAT cascade
transcription, DNA-templated
phosphorylation
serine phosphorylation of STAT3 protein
protein autophosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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