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Invitrogen
NR2F6 Polyclonal Antibody
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FIGURE: 1 / 3
NR2F6 Antibody (PA5-106467) in ICC/IF
Immunocytochemistry analysis of NR2F6 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NR2F6 polyclonal antibody (Product # PA5-106467) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106467
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NR2F6(Accession P10588), corresponding to amino acid residues K20-V70.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NR2F6.
Target Information
Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: avian erythroblastic leukemia viral (v-erb-a) oncogene homolog-like 2; COUP transcription factor 3; COUP-TF3; COUPg; EAR-2; Erba related 2; ERBA-related gene-2; Nuclear receptor subfamily 2 group F member 6; nuclear receptor V-erbA-related; orphan nuclear receptor EAR-2; Ovalbumin upstream promoter gamma nuclear receptor; ovalbumin upstream promoter gamma nuclear receptor rCOUPg; unnamed protein product; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2; V-erbA-related protein 2; V-erbA-related protein EAR-2
Gene Aliases: AV090102; COUP-TF3; EAR-2; EAR2; ERBAL2; NR2F6
UniProt ID: (Human) P10588, (Rat) O09017, (Mouse) P43136
Entrez Gene ID: (Human) 2063, (Rat) 245980, (Mouse) 13864
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
steroid hormone receptor activity
C4 zinc finger nuclear receptor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
nervous system development
cell differentiation
intracellular receptor signaling pathway
entrainment of circadian clock by photoperiod
neuron development
detection of temperature stimulus involved in sensory perception of pain
transcription, DNA-templated
transcription from RNA polymerase II promoter
steroid hormone mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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