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Invitrogen
GBL Polyclonal Antibody
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FIGURE: 1 / 2
GBL Antibody (PA5-30796) in WB
Western blot analysis of GBL in wild-type (WT) and mLST8 / GBL knockout (KO) HeLa cell extracts (30 µg). Samples were incubated with GBL polyclonal antibody (Product # PA5-30796) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG. Separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.
Product Details
PA5-30796
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human mLST8/GBL. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, 293T, A431, Jurkat, Raji.
Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (91%), Xenopus laevis (88%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G beta 6; G beta-like protein; guanine nucleotide binding protein; G protein beta subunit-like; Gable; GBL protein; Mammalian lethal with SEC13 protein 8; mLST8; mLST8 / mLST8; protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit gable; TORC subunit LST8; TORC subunit WAT1; unnamed protein product
Gene Aliases: GbetaL; GBL; LST8; MLST8; POP3; WAT1
UniProt ID: (Human) Q9BVC4
Entrez Gene ID: (Human) 64223
cellular response to DNA damage stimulus
cytoskeleton organization
negative regulation of autophagy
positive regulation of cell growth
positive regulation of actin filament polymerization
cellular response to nutrient levels
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
TORC1 signaling
TORC2 signaling
negative regulation of apoptotic process
protein kinase B signaling
positive regulation of glycolytic process
positive regulation of lipid biosynthetic process
positive regulation of protein kinase B signaling
cellular response to hypoxia
cellular response to osmotic stress
positive regulation of pentose-phosphate shunt
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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