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|Tested species reactivity||Human|
|Published species reactivity||Avian, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat recombinant PCNA polypeptides|
|Storage buffer||PBS with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Analyte Specific Reagent
Identification of prognostic protein biomarkers in childhood acute lymphoblastic leukemia (ALL).
PCNA01 was used in flow cytometry to identify prognostic protein biomarkers and potential regulators of prednisolone-induced apoptosis using leukemia cell lines.
|Jiang N,Kham SK,Koh GS,Suang Lim JY,Ariffin H,Chew FT,Yeoh AE||Journal of proteomics (74:843)||2011|
|Avian||Not Cited||Developmental origins of species differences in telencephalon and tectum size: morphometric comparisons between a parakeet (Melopsittacus undulatus) and a quail (Colinus virgianus).||Striedter GF,Charvet CJ||The Journal of comparative neurology (507:1663)||2008|
Enhanced survival and potent expansion of the natural killer cell population of HIV-infected individuals by exogenous interleukin-15.
PCNA01 was used in flow cytometry to assess the effects of IL-15 on proliferation and survival of CD56+ and CD16+ cells of HIV-infected individuals.
|Naora H,Gougeon ML||Immunology letters (68:359)||1999|
Cyclin; DNA polymerase delta auxiliary protein; PCNA; Proliferating cell nuclear antigen