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Invitrogen
PCNA Monoclonal Antibody (PC10)
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FIGURE: 1 / 80
PCNA Antibody (13-3900) in ICC/IF
Immunofluorescent analysis of PCNA was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PCNA Mouse Monoclonal Antibody (Product # 13-3900) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at roo... View More
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.jpg?time=20240507?time=20240507 "PCNA Antibody (13-3900) in IHC")
.jpg?time=20240507?time=20240507 "PCNA Antibody (13-3900) in IHC")
.jpg?time=20240507?time=20240507 "PCNA Antibody (13-3900) in IHC")
Product Details
13-3900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Insect,
Mouse,
Non-human primate,
Rat,
Yeast
Published species
Avian,
Bovine,
Fruit fly,
Guinea pig,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Shark,
Xenopus,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
PC10
Immunogen
Rat recombinant PCNA polypeptides.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; DNA sliding clamp PCNA; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen; unnamed protein product
Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR; POL30; YBR0811; YBR088C
UniProt ID: (Human) P12004, (Baker's yeast) P15873, (Rat) P04961, (Mouse) P17918
Entrez Gene ID: (Human) 5111, (Baker's yeast) 852385, (Rat) 25737, (Mouse) 18538
purine-specific mismatch base pair DNA N-glycosylase activity
DNA binding
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
macromolecular complex binding
DNA polymerase binding
transcription factor binding
DNA polymerase processivity factor
negative regulation of transcription from RNA polymerase II promoter
DNA replication
leading strand elongation
regulation of DNA replication
DNA repair
base-excision repair, gap-filling
mismatch repair
chromatin organization
cellular response to DNA damage stimulus
response to oxidative stress
heart development
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
response to estradiol
response to lipid
cellular response to UV
estrous cycle
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
cellular response to hydrogen peroxide
cellular response to xenobiotic stimulus
response to dexamethasone
liver regeneration
positive regulation of DNA-directed DNA polymerase activity
response to L-glutamate
mitotic telomere maintenance via semi-conservative replication
mitotic cell cycle
meiotic mismatch repair
lagging strand elongation
nucleotide-excision repair
postreplication repair
mitotic sister chromatid cohesion
chromatin silencing at silent mating-type cassette
telomeric heterochromatin assembly
establishment of mitotic sister chromatid cohesion
maintenance of DNA trinucleotide repeats
error-free translesion synthesis
positive regulation of exodeoxyribonuclease activity
positive regulation of phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands
positive regulation of exonuclease activity
regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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