Invitrogen

PDE4A Polyclonal Antibody

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PDE4A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PDE4A in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115730) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

PDE4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDE4A in mouse kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115730) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

PDE4A Antibody in Western Blot (WB) — Western blot analysis of PDE4A in HeLa cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115730).

Product Details

PA5-115730

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human PDE4A, corresponding to a region within C-terminal amino acids. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2900365

Target Information

Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction.

Communications biology

Fan T,Hou Y,Ge W,Fan T,Feng X,Guo W,Song X,Gao R,Wang J

PA5-115730 was used in Western Blot to indicate that rolipram, a PDE4 inhibitor, may be a potential drug for hypertension therapy.

Thu Jan 20 00:00:00 EST 2022

Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction.

Communications biology

Fan T,Hou Y,Ge W,Fan T,Feng X,Guo W,Song X,Gao R,Wang J

PA5-115730 was used in Western Blot to indicate that rolipram, a PDE4 inhibitor, may be a potential drug for hypertension therapy.

Thu Jan 20 00:00:00 EST 2022

Bioinformatics

Protein Aliases: cAMP-specific 3',5'-cyclic phosphodiesterase 4A; cAMP-specific phosphodiesterase PDE4A; cyclic AMP specific phosphodiesterase PDE4A5A; DPDE2; dunce; PDE46; phosphodiesterase 4A, cAMP specific; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila); phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4

Gene Aliases: D9Ertd60e; DPDE2; PDE4; PDE46; PDE4A; PHOSA

UniProt ID: (Human) P27815, (Mouse) O89084, (Rat) P54748

Entrez Gene ID: (Human) 5141, (Mouse) 18577, (Rat) 25638

Molecular Function: phosphodiesterase