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PI3 Polyclonal Antibody
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FIGURE: 1 / 2
PI3 Antibody (PA5-35877) in IHC (P)
PI3 Polyclonal Antibody detects Elafin protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PI3 Polyclonal Antibody (Product # PA5-35877) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35877
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Elafin. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
Product Specific Information
Recommended positive controls: human PI3-transfected 293T.
Predicted reactivity: Rhesus Monkey (92%), Chimpanzee (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Peptidase inhibitor 3 (PI3), also known as Elafin, is a gene located on chromosome 20q13.12, encoding a protein that functions as a serine protease inhibitor. Elafin is primarily expressed in epithelial tissues, including skin and mucosal surfaces, where it plays a protective role against excessive proteolytic activity by inhibiting enzymes like neutrophil elastase and proteinase 3. This activity is crucial for maintaining tissue homeostasis and preventing inflammatory damage. PI3 is regulated by pro-inflammatory cytokines and is involved in modulating inflammatory responses and tissue repair processes. Its expression is often elevated in conditions characterized by inflammation, such as chronic obstructive pulmonary disease (COPD), psoriasis, and other inflammatory diseases, reflecting its role in guarding against protease-induced tissue damage. Research into PI3 focuses on its potential therapeutic applications in inflammatory diseases and its utility as a biomarker for assessing inflammation-related pathologies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: elastase-specific inhibitor; peptidase inhibitor 3, skin-derived; PI-3; PI3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); protease inhibitor WAP3; skin-derived antileukoproteinase; trappin-2; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14
Gene Aliases: cementoin; ESI; SKALP; WAP3; WFDC14
UniProt ID: (Human) P19957
Entrez Gene ID: (Human) 5266
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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