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Agrisera
PPD2/Protein PEAPOD 2 Polyclonal Antibody
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FIGURE: 1 / 1
PPD2/Protein PEAPOD 2 Antibody (AS15-3107) in WB
Equal volumes of total proteins from E. coli purified with GST agarose beads or N. benthamiana were extracted and denatured with LaemLli buffer (50 m M Tris p H 8,10 m M EDTA, 18% glycerol, and 10 m M DTT) at 95°C for 10 min. Samples were separated on 12% SDS-PAGE and blotted 30 min to Protran nitrocellulose membrane (Whatman) using semi-dry transfer. Blots were blocked with 0.25% BSA/0.25% gelatin for 60 m... View More
Product Details
AS15-3107
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana PPD2 sequence.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
PPD2 is a plant nuclear gene found in Arabidopsis thaliana within the PEAPOD locus, producing a plant-specific transcriptional regulator that functions as a DNA-associated repressor controlling lateral organ growth and morphology. The encoded PPD2 protein is characterized by a conserved TIFY family motif and a divergent CCT-like region consistent with a regulatory, non-enzymatic role, and genetic evidence supports functional redundancy with its paralog in restricting prolonged dispersed meristematic cell proliferation during leaf blade development. Loss of PPD2 activity contributes to enlarged, dome-shaped leaves and altered silique shape, consistent with a role in limiting lamina expansion and coordinating growth across the organ surface; mechanistically, PPD2 participates in transcriptional control of cell-cycle programs, including repression of CYCLIN D3 gene expression in concert with NINJA, thereby promoting timely arrest of cell division that is required for leaf flatness and appropriate organ size.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT4G14720; DL3400C; FCAALL.297; PEAPOD 2; TIFY4B
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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