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CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red
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FIGURE: 1 / 4
CD11b Antibody (RM2817) in Flow
Stem cell research & therapy 2024 -
Reduced infiltration of bone marrow-derived macrophages in the retina of diabetic NOD1 chimeras. A Flow cytometry gating strategy for Ly6C lo and Ly6C hi monocytes in bone marrow. B Flow cytometry diagrams of bone marrow monocytes from WT - WT, NOD1 -/- - WT, Akita - Akita and NOD1 -/- -Akita - Akita mice. C , D The percentages of Ly6C lo and Ly6C hi monocytes among CD45 + cells (flow cytometry analysis; n ... View More
Product Details
RM2817
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
M1/70.15
Immunogen
C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes
Conjugate
Excitation/Emission Max
567/615 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS with sucrose, 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The M1/70.15 monoclonal antibody (mAb) reacts with the alpha-M subunit of Mac-1 (CD11b/CD18). For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm). Applications for the M1/70.15 mAb include immunoprecipitation, immunostaining for flow cytometry, IHC of acetone-fixed frozen sections, and blocking of CR3-mediated rosetting.
Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Every lot is tested by flow cytometry using freshly harvested mouse blood.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044
Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1
Entrez Gene ID: (Mouse) 16409
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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