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          • Primary Antibodies ›
          • RUNX1/RUNX2 Antibodies

          Bioss

          RUNX1/RUNX2 Polyclonal Antibody

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          Cite RUNX1/RUNX2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          RUNX1/RUNX2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          RUNX1/RUNX2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          RUNX1/RUNX2 Antibody (BS-1134R) in WB

          Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse heart lysates; Lane 4: Rat heart lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RUNX1/RUNX2 Antibody in Western Blot (WB)
          RUNX1/RUNX2 Antibody in Western Blot (WB)

          Product Details

          BS-1134R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:300
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:400
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          Flow Cytometry (Flow)

          1:20-1:100
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Guinea pig, Human, Mouse, Rabbit, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide derived from human RUNX2.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Acute myeloid leukemia 1 protein; Acute myeloid leukemia 3 protein; AKV core binding factor; aml1; AML1-ETO fusion; AML1-ETO fusion protein; AML1-EVI-1 fusion protein; CBF-alpha-1; CBF-alpha-2; core binding factor alpha 1; core binding factor alpha 2; core-binding factor runt domain alpha subunit 2 (acute myeloid leukemia 1 oncogene); Core-binding factor subunit alpha-1; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 1; core-binding factor, runt domain, alpha subunit 2; mutant RUNX1; Oncogene AML-1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEA2-alpha B; PEBP2 alpha A; PEBP2-alpha A; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha A subunit; Polyomavirus enhancer-binding protein 2 alpha B subunit; runt domain, alpha subunit 1; runt domain, alpha subunit 2; runt related transcription factor 1; runt related transcription factor 2; runt-related factor 2; Runt-related transcription factor 1; Runt-related transcription factor 2; RUNX1 with skipped exon 5; SL3-3 enhancer factor 1 alpha A subunit; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha A subunit; SL3/AKV core-binding factor alpha B subunit

          View more View less

          Gene Aliases: AML1; AML1-EVI-1; AML3; AMLCR1; Cbf; CBF-alpha-1; CBF-alpha-2; CBF2alpha; Cbfa-1; CBFA1; CBFA2; CCD; CCD1; CLCD; EVI-1; LS3; OSF-2; OSF2; PEA2aA; PEBP2A; Pebp2a1; Pebp2a2; PEBP2aA; PEBP2aB; PEBP2alpha; Pebpa2a; Pebpa2b; RUNX1; RUNX2

          View more View less

          UniProt ID: (Human) Q01196, (Human) Q13950, (Rat) Q63046, (Mouse) Q08775

          View more View less

          Entrez Gene ID: (Human) 861, (Rabbit) 100358993, (Rabbit) 100008943, (Human) 860, (Guinea pig) 100724149, (Rat) 50662, (Mouse) 12394, (Rat) 367218, (Guinea pig) 100734175, (Mouse) 12393

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding protein binding ATP binding protein homodimerization activity protein heterodimerization activity DNA-binding transcription factor binding sequence-specific double-stranded DNA binding core promoter proximal region sequence-specific DNA binding chromatin binding protein domain specific binding chromatin DNA binding bHLH transcription factor binding regulatory region DNA binding core promoter binding molecular_function transcription factor binding repressing transcription factor binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding histone deacetylase binding transcription regulatory region DNA binding Runt transcription factor DNA-binding transcription factor immunoglobulin fold transcription factor gene-specific transcriptional regulator
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter ossification chondrocyte differentiation myeloid leukocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of plasminogen activation hemopoiesis myeloid cell differentiation neuron differentiation negative regulation of granulocyte differentiation positive regulation of granulocyte differentiation positive regulation of interleukin-2 production positive regulation of collagen biosynthetic process negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of CD8-positive, alpha-beta T cell differentiation regulation of cell differentiation positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter peripheral nervous system neuron development positive regulation of developmental process positive regulation of multicellular organismal process regulation of cardiac muscle cell proliferation cardiac muscle tissue regeneration hematopoietic stem cell proliferation positive regulation of extracellular matrix organization regulation of connective tissue replacement regulation of multicellular organismal development skeletal system development osteoblast differentiation endochondral ossification osteoblast fate commitment chondrocyte development osteoblast development transmembrane receptor protein serine/threonine kinase signaling pathway smoothened signaling pathway cell proliferation positive regulation of cell proliferation gene expression regulation of gene expression positive regulation of gene expression cell differentiation T cell differentiation regulation of ossification bone mineralization BMP signaling pathway positive regulation of chondrocyte differentiation embryonic forelimb morphogenesis ligamentous ossification regulation of fibroblast growth factor receptor signaling pathway odontogenesis of dentin-containing tooth regulation of odontogenesis of dentin-containing tooth regulation of osteoblast differentiation positive regulation of osteoblast differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated cell maturation embryonic cranial skeleton morphogenesis skeletal system morphogenesis stem cell differentiation epithelial cell proliferation positive regulation of epithelial cell proliferation SMAD protein signal transduction stem cell proliferation response to sodium phosphate positive regulation of stem cell proliferation in utero embryonic development liver development myeloid progenitor cell differentiation transcription, DNA-templated central nervous system development negative regulation of cell proliferation regulation of signal transduction hair follicle morphogenesis response to retinoic acid embryonic hemopoiesis behavioral response to pain neuron development definitive hemopoiesis regulation of hair follicle cell proliferation cellular response to transforming growth factor beta stimulus positive regulation of cell maturation positive regulation of progesterone secretion regulation of T cell anergy transcription from RNA polymerase II promoter positive regulation of interferon-gamma production cellular response to fibroblast growth factor stimulus positive regulation of ossification cellular response to BMP stimulus positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
          It has to be done as per old AB suggested Products section.

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