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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine5, eBioscience™
Product Details
15-4301-82
| Applications | Tested Dilution |
|---|---|
Flow Cytometry (Flow) |
Assay-Dependent |
Control (Ctrl) |
Assay-Dependent |
| Product Specifications | |
|---|---|
Host/Isotype |
Rat / IgG1, kappa |
Class |
Control |
Type |
Isotype Control |
Clone |
eBRG1 |
Conjugate |
PE-Cyanine5
|
Excitation/Emission Max |
568/666 nm
View spectra
|
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_470136 |
Product Specific Information
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: PE-Cy5 Rat IgG1 Iso Cntrl has been reported for use in flow cytometric analysis.
Applications Tested: This PE-Cy5 Rat IgG1 isotype control antibody should be used at the same concentration as the experimental antibody.
Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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