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Invitrogen
Applications | Tested Dilution |
---|---|
Immunohistochemistry (IHC) |
Assay-Dependent |
Immunocytochemistry (ICC/IF) |
1 µg/mL |
Control (Ctrl) |
Assay-Dependent |
Product Specifications | |
---|---|
Host/Isotype |
Rat / IgG1, kappa |
Class |
Control |
Type |
Isotype Control |
Clone |
eBRG1 |
Conjugate |
eFluor™ 570
|
Excitation/Emission Max |
556/569 nm View spectra |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_11220080 |
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This eBRG1 antibody has been reported for use in immunohistochemical and immunocytochemical staining.
Applications Tested: This eBRG1 antibody has been tested by immunohistochemistry on frozen mouse tissue. Use isotype control at the same concentration as experimental antibody.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
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