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Bioss
SHARPIN Polyclonal Antibody
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FIGURE: 1 / 1
SHARPIN Antibody (BS-9581R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SHARPIN Polyclonal Antibody, Unconjugated (bs-9581R) at 1:400 overnight at 4°C, followed by DAB staining.
Product Details
BS-9581R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human SHARPIN, amino acids 260-310.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSIPL1; mSIPL1; protein kinase C-interacting protein RBCC like 1; SHANK-associated RH domain interacting protein; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; shank-interacting protein; Shank-interacting protein-like 1; Sharpin
Gene Aliases: 0610041B22Rik; AW121341; Conneck1; Cpdm; PSEC0216; RBCKL1; SHARPIN; SIPL1
UniProt ID: (Human) Q9H0F6, (Rat) Q9EQL9, (Mouse) Q91WA6
Entrez Gene ID: (Human) 81858, (Rat) 81859, (Mouse) 106025
mitochondrion organization
I-kappaB kinase/NF-kappaB signaling
brain development
regulation of tumor necrosis factor-mediated signaling pathway
apoptotic nuclear changes
keratinization
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of inflammatory response
protein homooligomerization
protein linear polyubiquitination
regulation of CD40 signaling pathway
epidermis development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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