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Proteintech
SMAD2 Polyclonal Antibody
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Product Details
12570-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Plant,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SMAD2 Fusion Protein Ag3237 (1-355 aa encoded by BC014840)
Conjugate
Form
Liquid
Concentration
0.22 mg/mL
Amount
33 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa.
Immunogen sequence: MSSILPFTP PVVKRLLGWK KSAGGSGGAG GGEQNGQEEK WCEKAVKSLV KKLKKTGRLD ELEKAITTQN CNTKCVTIPS TCSEIWGLST PNTIDQWDTT GLYSFSEQTR SLDGRLQVSH RKGLPHVIYC RLWRWPDLHS HHELKAIENC EYAFNLKKDE VCVNPYHYQR VETPVLPPVL VPRHTEILTE LPPLDDYTHS IPENTNFPAG IEPQSNYIPE TPPPGYISED GETSDQQLNQ SMDTGSPAEL SPTTLSPVNH SLDLQPVTYS EPAFWCSIAY YELNQRVGET FHASQPSLTV DGFTDPSNSE RFCLGLLSNV NRNATVEMTR RHIGRGVRLY YIGGEVFAEC LSDSAI (1-355 aa encoded by B C014840)
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3; unnamed protein product
Gene Aliases: 7120426M23Rik; CHTD8; hMAD-2; hSMAD2; JV18; JV18-1; LDS6; MADH2; MADR2; mMad2; Smad-2; SMAD2
UniProt ID: (Human) Q15796, (Mouse) Q62432, (Rat) O70436
Entrez Gene ID: (Human) 4087, (Mouse) 17126, (Rat) 29357
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
phosphatase binding
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
SMAD binding
metal ion binding
tau protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
disordered domain specific binding
DNA-binding transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
activating transcription factor binding
protein homodimerization activity
protein heterodimerization activity
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
in utero embryonic development
endoderm formation
mesoderm formation
regionalization
determination of left/right asymmetry in lateral mesoderm
aortic valve morphogenesis
pulmonary valve morphogenesis
endocardial cushion morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transforming growth factor beta receptor signaling pathway
zygotic specification of dorsal/ventral axis
gastrulation
pattern specification process
endoderm development
mesoderm development
heart development
cell proliferation
anatomical structure morphogenesis
response to glucose
post-embryonic development
embryonic pattern specification
anterior/posterior pattern specification
positive regulation of gene expression
negative regulation of gene expression
positive regulation of epithelial to mesenchymal transition
regulation of transforming growth factor beta receptor signaling pathway
insulin secretion
cell differentiation
negative regulation of ossification
lung development
positive regulation of BMP signaling pathway
pancreas development
primary miRNA processing
activin receptor signaling pathway
organ growth
intracellular signal transduction
nodal signaling pathway
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
developmental growth
embryonic foregut morphogenesis
embryonic cranial skeleton morphogenesis
regulation of multicellular organismal process
pericardium development
SMAD protein signal transduction
mesenchyme development
trophoblast cell migration
secondary palate development
response to cholesterol
response to transforming growth factor beta
odontoblast differentiation
transcription from RNA polymerase II promoter
protein phosphorylation
common-partner SMAD protein phosphorylation
SMAD protein complex assembly
negative regulation of cell proliferation
signal transduction involved in regulation of gene expression
regulation of binding
palate development
positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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