Invitrogen

TRBP Polyclonal Antibody

Product Image Gallery

TRBP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of HeLa cells using PA5-19716, anti-TRBP antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 ug/ml overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).

TRBP Antibody in Western Blot (WB) — Western blot analysis of A431 Whole Cell Lysate using PA5-19716, TRBP primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19716 staining of MCF7 Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Product Details

PA5-19716

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunocytochemistry (ICC/IF)	1 µg/mL	-
Immunoprecipitation (IP)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human TRBP.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10983644

Product Specific Information

This antibody is predicted to react with mouse and cow based on sequence homology.

Target Information

TRBP belongs to the family of dsRNA-binding proteins. It consists of highly conserved 2 dsRNA-binding domains and C-terminal basic region. It is a unique cellular protein capable of binding to HIV-TAR RNA and stimulates expression of HIV-1 long terminal repeats. It also inhibits the host antiviral and anti-proliferative mechanisms by directly binding to IFN-regulated dsRNA-dependent protein kinase (PKR). TRBP associates with Ago2 protein and forms an integral component of Dicer-containing complex. It has a role in micro RNA processing and in RISC assembly. Other functions of TRBP include promoting cellular growth and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: RISC-loading complex subunit TARBP2; RISC-loading complex subunit TARBP2 {ECO:0000255|HAMAP-Rule:MF_03034}; TAR (HIV) RNA binding protein 2; TAR (HIV) RNA-binding protein 2; TAR (HIV) RNA-binding protein TRBP1; TAR (HIV-1) RNA binding protein 2; TAR RNA binding protein 2; TAR RNA-binding protein 2; TARBP2; trans-activation responsive RNA-binding protein; Trans-activation-responsive RNA-binding protein

Gene Aliases: TARBP2; TRBP