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UBE2W Polyclonal Antibody
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FIGURE: 1 / 1
UBE2W Antibody (BS-8385R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with UBE2W Polyclonal Antibody, Unconjugated (bs-8385R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product Details
BS-8385R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human UBE2W, amino acids 71-151.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins, and catalyzes monoubiquitination. Further, UBE2W is involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. UBE2W In vitro catalyzes 'Lys-11'-linked polyubiquitination, and transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 ubiquitin-conjugating enzyme W; FLJ11011; N-terminal E2 ubiquitin-conjugating enzyme; N-terminus-conjugating E2; probable ubiquitin-conjugating enzyme E2 W; UBC-16; UBC16; Ubiquitin carrier protein W; ubiquitin conjugating enzyme E2 W (putative); ubiquitin conjugating enzyme E2W (putative); Ubiquitin-conjugating enzyme 16; ubiquitin-conjugating enzyme 2W; Ubiquitin-conjugating enzyme E2 W; ubiquitin-conjugating enzyme E2 W-like; ubiquitin-conjugating enzyme E2W; Ubiquitin-protein ligase W; unnamed protein product
Gene Aliases: 6130401J04Rik; UBC-16; UBC16; UBE2W
UniProt ID: (Human) Q96B02, (Mouse) Q8VDW4, (Rat) B5DEI4
Entrez Gene ID: (Human) 55284, (Mouse) 66799, (Rat) 100909445
protein polyubiquitination
DNA repair
protein monoubiquitination
misfolded or incompletely synthesized protein catabolic process
cellular response to DNA damage stimulus
protein ubiquitination
cellular response to nutrient levels
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K11-linked ubiquitination
cellular response to misfolded protein
antiviral innate immune response
negative regulation of TORC1 signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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