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Invitrogen
UBE2W Polyclonal Antibody
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FIGURE: 1 / 3
UBE2W Antibody (PA5-71833) in IHC (P)
Immunohistochemistry analysis of UBE2W in formalin fixed and paraffin embedded human liver tissue. Samples were incubated with UBE2W polyclonal antibody (Product # PA5-71833) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Product Details
PA5-71833
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins, and catalyzes monoubiquitination. Further, UBE2W is involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. UBE2W In vitro catalyzes 'Lys-11'-linked polyubiquitination, and transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 ubiquitin-conjugating enzyme W; N-terminal E2 ubiquitin-conjugating enzyme; N-terminus-conjugating E2; probable ubiquitin-conjugating enzyme E2 W; UBC-16; Ubiquitin carrier protein W; ubiquitin conjugating enzyme E2W (putative); Ubiquitin-conjugating enzyme 16; ubiquitin-conjugating enzyme 2W; Ubiquitin-conjugating enzyme E2 W; ubiquitin-conjugating enzyme E2 W-like; ubiquitin-conjugating enzyme E2W; ubiquitin-conjugating enzyme E2W (putative); Ubiquitin-protein ligase W
Gene Aliases: 6130401J04Rik; UBC-16; UBC16; UBE2W
UniProt ID: (Human) Q96B02, (Mouse) Q8VDW4
Entrez Gene ID: (Human) 55284, (Mouse) 66799, (Rat) 100909445
DNA repair
protein monoubiquitination
misfolded or incompletely synthesized protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K11-linked ubiquitination
cellular response to misfolded protein
protein polyubiquitination
cellular response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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