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Bioss

UHRF1/2 Polyclonal Antibody

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Product Details

BS-6427R

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:20-1:100
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human UHRF1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. Tissue specificity: Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. UHRF2 is an E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. E3 SUMO-, but not ubiquitin-, protein ligase for ZNF131.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase UHRF1; E3 ubiquitin-protein ligase UHRF2; hUHRF1; HuNp95; inverted CCAAT box binding protein 90; Inverted CCAAT box-binding protein of 90 kDa; Liver regeneration-related protein LRRG126; mUhrf1; NIRF; Np95-like RING finger protein; Np95/ICBP90-like RING finger protein; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear protein 97; Nuclear zinc finger protein Np95; Nuclear zinc finger protein Np97; RING finger protein 106; RING finger protein 107; RING-type E3 ubiquitin transferase UHRF1; RING-type E3 ubiquitin transferase UHRF2; thymus and cell proliferation associated protein; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; Ubiquitin-like PHD and RING finger domain-containing protein 2; ubiquitin-like with PHD and ring finger domains 2, E3 ubiquitin protein ligase; ubiquitin-like, containing PHD and RING finger domains 2; ubiquitin-like, containing PHD and RING finger domains, 1; ubiquitin-like, containing PHD and RING finger domains, 2; Ubiquitin-like-containing PHD and RING finger domains protein 1; Ubiquitin-like-containing PHD and RING finger domains protein 2; unnamed protein product

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Gene Aliases: 2310065A22Rik; Ac2-121; AI426270; AL022808; AW214556; D130071B19Rik; hNP95; hUHRF1; huNp95; ICBP90; NIRF; NP95; RNF106; RNF107; TDRD22; TDRD23; UHRF1; UHRF2; URF2

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UniProt ID: (Human) Q96T88, (Human) Q96PU4, (Mouse) Q8VDF2, (Rat) Q7TPK1, (Mouse) Q7TMI3

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Entrez Gene ID: (Human) 29128, (Human) 115426, (Mouse) 18140, (Rat) 316129, (Rat) 309331, (Mouse) 109113

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Function(s)
core promoter proximal region sequence-specific DNA binding nucleic acid binding DNA binding ubiquitin-protein transferase activity protein binding zinc ion binding methyl-CpG binding transferase activity histone binding identical protein binding hemi-methylated DNA-binding metal ion binding ubiquitin protein ligase activity histone H3K9me2/3 reader activity DNA damage sensor activity histone H3 ubiquitin ligase activity SUMO transferase activity RNA polymerase II sequence-specific DNA binding transcription factor binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity nucleosomal histone binding methylated histone binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity molecular_function ubiquitin-protein ligase
Process(es)
negative regulation of transcription from RNA polymerase II promoter double-strand break repair via homologous recombination DNA repair chromatin organization ubiquitin-dependent protein catabolic process response to stress cellular response to DNA damage stimulus protein ubiquitination heterochromatin assembly reciprocal DNA recombination regulation of gene expression, epigenetic hypermethylation of CpG island positive regulation of transcription from RNA polymerase II promoter regulation of epithelial cell proliferation positive regulation of protein metabolic process protein autoubiquitination mitotic spindle assembly regulation of transcription from RNA polymerase II promoter protein sumoylation cell differentiation regulation of cell cycle transcription, DNA-templated regulation of transcription, DNA-templated cell cycle cell proliferation maintenance of DNA methylation histone monoubiquitination chromatin modification histone ubiquitination positive regulation of cellular protein metabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of methylation-dependent chromatin silencing response to peptide hormone biological_process positive regulation of cell cycle arrest
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