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WFDC2 Antibody (PA5-80226) in IHC (P)
Immunohistochemistry analysis of WFDC2 on paraffin-embedded rat small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with WFDC2 polyclonal antibody (Product# PA5-80226) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Deve... View More
Product Details
PA5-80226
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human HE4 recombinant protein (Position: E31-F124).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747340
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human MDA-MB-231 whole cell, human MDA-MB-453 whole cell, rat thymus tissue, mouse testis tissue, mouse thymus tissue. IHC: rat small intestine tissue, mouse spleen tissue, rat spleen tissue.
Target Information
This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymal protein 4; Epididymal secretory protein 4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; Major epididymis-specific protein E4; MGC57529; Putative protease inhibitor WAP5; RE4; WAP domain containing protein HE4-V4; WAP domain-containing protein HE4; WAP four-disulfide core domain protein 2
Gene Aliases: 1600023A02Rik; dJ461P17.6; EDDM4; HE4; re4; WAP5; WFDC2
UniProt ID: (Human) Q14508, (Rat) Q8CHN3, (Mouse) Q9DAU7
Entrez Gene ID: (Human) 10406, (Rat) 286888, (Mouse) 67701
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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