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Take advantage of our advanced data analysis features
AccuSEQ real-time PCR software is meticulously crafted to enhance the data interpretation of vital tests necessary for GMP manufacturing. It helps simplify the analysis of in-process testing and lot-release testing, supporting precise and efficient results. With its advanced features, AccuSEQ software enables easy integration into your manufacturing workflow, with reliable insights that support the highest standards of quality and compliance.
- An integrated, easy-to-use interface to enable consistency across multiple data applications, reducing the time needed to train your team
- A single software platform to support all current and future resDNASEQ, MycoSEQ, and ViralSEQ real-time PCR assays, as well as custom experiments
- Security, audit, and e-signature (SAE) capabilities to support 21 CFR Part 11 compliance
- Availability for QuantStudio 7 Pro (NEW), QuantStudio 5, and 7500 Fast systems for easy integration of SEQ solutions into your current QC laboratory
- Flexible throughput of 10–500 samples per week
- Complete traceability with assay-specific output, helping eliminate manual calculations
- Comprehensive data management and reporting features
What’s new in v4.0
AccuSEQ software v4.0—and its compatibility with QuantStudio qPCR systems—offers new capabilities that enable you to develop an integrated, efficient, and cost-effective approach to data interpretation for contaminant and impurity testing.
- Allows easy instrument runs and analysis with QuantStudio 7 Pro and QuantStudio 5 systems to provide virtually uninterrupted workflows, regardless of instrument platform
- Supports data analyses for QuantStudio 6 Flex and QuantStudio 7 Flex systems to help leverage current instrument investments, maintain existing processes, and control costs
- Helps simplify setup and enable time savings with templates for SEQ analytical assays that easily convert default templates (QuantStudio 5 0.1 mL) for all other instruments and blocks
- Supports greater productivity with QuantStudio 7 Pro flexible block formats that can be changed without needing to switch instruments
- Enables precise and reliable performance assessment with the enhanced calibration tracking of QuantStudio 7 Pro systems
- Protects your budget by making easy assay updates and importing new templates for factory-validated SEQ analytical assays without upgrading the software
- Promotes greater speed and user productivity with a new user interface and enhanced SAE module
- Plate views and visualization
- Pipeline analysis settings
- Sample setup import
Updated plate views and a plus/minus view support enhanced data visualization for faster assessments and more comprehensive and visually appealing reports.
Help achieve data accordance with previous AccuSEQ software versions and QC alerts with advanced settings that support multiple analyses.
Designed for easier sample setup with sample types, sample type references, and setup information available from a LIMS using a .csv file.
Part of your complete workflow
Thermo Fisher Scientific offers a comprehensive solution for the entire analytical workflow, encompassing sample preparation, SEQ assays, qPCR instruments, and AccuSEQ software. During sample preparation, you can achieve reliable and consistent recovery of target DNA and RNA with trusted PrepSEQ chemistry. Utilize a variety of assays, including resDNASEQ, MycoSEQ, and ViralSEQ, designed to detect impurities, identify contamination, and quantify titers. AccuSEQ software then runs these assays on QuantStudio 7 Pro and QuantStudio 5 instruments to analyze the data and help deliver actionable insights. Unifying your sample preparation, assays, and instrumentation on a single software platform offers you the control to tune your workflow to your specific requirements.
The right solution at the right time
AccuSEQ software v4.0 helps advance your capabilities, improve productivity, and realize cost efficiencies no matter where you are in your testing evolution. See how you can benefit based on your current testing stage:
| Adopting qPCR technology or upgrading custom qPCR software and instruments | Existing AccuSEQ software users upgrading to new qPCR instrumentation | Recently upgraded to a new qPCR instrument, but require compatible software |
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Getting the right start
The AccuSEQ software v4.0 dashboard is intuitive and user-friendly. Use the home page to start a new experiment, get immediate updates for ongoing experiments, and access quick links to the most recent experiments. Find your most recent experiments under “Home.” Check the status and time remaining of your real-time PCR run under “Monitor The Run.” Browse through factory default templates (validated and optimized templates for assays, including MycoSEQ and resDNASEQ), user-saved templates, or create custom templates tailored to your needs under “Create New Experiment.”
Key features
- Plate setup
- Automated analysis
- Flexibility
- Predefined templates
The AccuSEQ software v4.0 plate setup interface provides a visual approach to setting up experiment details on a 96-well plate. Input details—sample, controls, dilutions, and more—and designate colors for each well, allowing for quick visualization of the 96-well layout. Table-view plate setup is also available. The “Define & Setup Standard” feature allows you to expedite standards setup on the plate. Simply enter information about the target and standard curve parameters, and this feature provides a well-arranged plate for the standards.
AccuSEQ software features automated quantitation analysis and presence/absence calls based on a decision tree that utilizes parameters such as Ct, IPC Diff, and Delta Rn. It also features automated detection of PCR inhibition in sample wells.
AccuSEQ software allows for great flexibility with experiments through options to modify call settings to meet individual specifications. This feature allows you to modify the thresholds for every call built as part of the decision tree in a presence/absence call. AccuSEQ software can also review calls to be overridden. This can be done when you do not agree with the system calls and want to override it with your own assessment.
Predefined templates allow for a streamlined experiment setup, where factory default templates for Thermo Fisher Scientific SEQ products get you up and running faster.
Ordering information
Analytics Knowledge Hub
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For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.