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Nimesh Khadka, PhD

Expertise

Analytical ChemistrybiochemistrychemometricsPATprocess development

Education

Utah State University - Ph.D , Biochemistry; Universal Science College, Nepal B.S, Biochemistry

Nimesh Khadka, PhD

Sr Product Applications Specialist, Thermo Fisher Scientific

Nimesh Khadka is a senior application scientist at Thermo Fisher Scientific, specializing in analytical biochemistry, spectroscopy, and AI/ML-based chemometrics. Passionate about innovation, he leads efforts in utilizing Raman spectroscopy as a process analytical technology (PAT) for monitoring, control, and automation of bioprocesses, industrial processes, and chemical reactions.

Nimesh earned his Ph.D. in analytical biochemistry from Utah State University, focusing on complex metallo-enzymes using vibrational (Raman and FT-IR) and other spectroscopic techniques like UV-Vis, EPR/ENDOR, NMR coupled with mathematical modeling and simulation. He completed postdoctoral training at Case Western Reserve University, researching structural biology in biomolecules related to eye pathologies. This extensive background has given him a deep understanding of bioprocesses, structure-function relationships, and deployment of analytical tools for diverse applications. Nimesh has published and coauthored high-impact factor journals such as Science, JACS, PNAS, and Inorganic Chemistry.

An active member of the American Chemical Society, Nimesh stays at the forefront of emerging technologies and is committed to driving innovation to meet customer needs.

Accomplishments

• FSI Innovation Ideation Winner (2022). Thermo Fisher Scientific • R&D Innovation Award (2021), Millipore Sigma • Media coverages on three of the scientific findings carried out with Pacific Northwest National Laboratory (PNNL) for Carbon Capture Utilization and Storage (CCUS) and National Renewable Energy Laboratory (NREL) for renewable and clean energy production. • Thomas F. Emery Outstanding Graduate Student in Biochemistry (2016), Utah State University • Gold medalist (2008), Pokhara University, Nepal • Dean’s List Student (2008), Pokhara University, Nepal. • Research Initiatives Winner (2008), Yonsei University (visiting undergraduate researcher) • Undergraduate academic scholarship, Pokhara University, Kathmandu, Nepal (2004-2008)

Publications

  • KA Brown, DF Harris, MB Wilker, A Rasmussen, N Khadka, H Hamby, (2016/4/22). Light-driven dinitrogen reduction catalyzed by a CdS: nitrogenase MoFe protein biohybrid. American Association for the Advancement of Science, 448-450.
  • Nimesh Khadka, Ross D. Milton, Sudipta Shaw, Dmitriy Lukoyanov, Dennis R. Dean, Shelley D. Minteer, Simone Raugei, Brian M. Hoffman, Lance C. Seefeld (August 29, 2017). Mechanism of Nitrogenase H2 Formation by Metal-Hydride Protonation Probed by Mediated Electrocatalysis and H/D Isotope Effects. Journal of the American Chemical Society, 139, 38, 13518–13524.
  • RD Milton, A Sofiene, N Khadka, DR Dean, D Leech, LC Seefeldt, (2016). Nitrogenase bioelectrocatalysis: heterogeneous ammonia and hydrogen production by MoFe protein. Energy and Environmental Science, 2250-2554.
  • D Lukoyanov, ZY Yang, N Khadka, DR Dean, LC Seefeldt, BM Hoffman (2016). Reductive Elimination of H2 Activates Nitrogenase to Reduce the NN Triple Bond: Characterization of the E4(4H) Janus Intermediate in Wild-Type Enzyme. Journal of the American Chemical Society , 3610-3615.

Advancing Materials

Different types of used plastic packaging arranged on a yellow background

K 2025: Showcasing the Future of Sustainable, Smart, and Responsible Plastics Production

October 9, 2025

Read more K 2025: Showcasing the Future of Sustainable, Smart, and Responsible Plastics Production

Behind the Bench

concepts of Experiments in the laboratory

Enhancing Biomanufacturing Automation with Process Raman Spectroscopy – A Quick Guide

September 16, 2025

Read more Enhancing Biomanufacturing Automation with Process Raman Spectroscopy – A Quick Guide
Tablets and Capsules Manufacturing Process. Close-up Shot of Medical Drug Production Line. White Painkiller Pills are Moving on Conveyor at Modern Pharmaceutical Factory.

In-line Raman Spectroscopy Pairs with Hot-Melt Extrusion in Pharmaceutical Manufacturing

August 19, 2025

Read more In-line Raman Spectroscopy Pairs with Hot-Melt Extrusion in Pharmaceutical Manufacturing
double helix dna abstract background

Scaling Nucleic Acid Therapeutic Manufacturing: The Promise of Raman

August 12, 2025

Read more Scaling Nucleic Acid Therapeutic Manufacturing: The Promise of Raman

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