With the Thermo Scientific CellInsight CX7 high-content instrument, you can take advantage of the entire fluorescent spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Use the tables below to select reagents for each channel; they are divided into application areas, including products for segmentation, cell structure, and more.

  • Select the Fixed cells or Live/fixable cells tab to see a reagent selection that matches your detection protocol.
  • Click on individual product links (within colored cells) to see product details including spectral profiles, images, sample data sets, citations, and cell-based protocols.
  • Click on an application area to see selection guides for additional fluorescence-based tools and assays for imaging.
Blue Cyan Green/Yellow Orange Red Deep Red Near IR

Antibody labels for secondary detection

Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 750
    Goat anti-chicken IgY   Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY  
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
Goat anti-rat IgG   Goat anti-rat IgG   Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG  
    Goat anti-guinea pig IgG   Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG  
    Goat anti-human IgG   Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG  
Donkey anti-goat IgG   Donkey anti-goat IgG   Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG  
    Donkey anti-mouse IgG   Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG  
    Donkey anti-rat IgG     Donkey anti-rat IgG    
    Rabbit anti-goat IgG   Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG  
Rabbit anti-mouse IgG   Rabbit anti-mouse IgG   Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG  
    Rabbit anti-rat IgG     Rabbit anti-rat IgG    
    Chicken anti-goat IgG     Chicken anti-goat IgG Chicken anti-goat IgG  
    Chicken anti-mouse IgG     Chicken anti-mouse IgG Chicken anti-mouse IgG  
    Chicken anti-rabbit IgG     Chicken anti-rabbit IgG Chicken anti-rabbit IgG  
    Chicken anti-rat IgG     Chicken anti-rat IgG Chicken anti-rat IgG  

Cell segmentation

Whole cell segmentation
HCS CellMask Blue   HCS CellMask Green   HCS CellMask Orange   HCS CellMask Deep Red  
Nuclear segmentation
HCS NuclearMask  Blue   SYTO 9 Green fluorescent nucleic acid stain   SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red  
DAPI   SYTOX Green nucleic acid stain     HCS NuclearMask Red TO-PRO-3 stain  

Cell structure

Cytoskeleton—actin
Alexa Fluor 350 phalloidin   Alexa Fluor 488 phalloidin   Alexa Fluor 555 phalloidin Alexa Fluor 594 phalloidin Alexa Fluor 647 phalloidin  
Plasma membrane
Wheat germ agglutinin, Alexa Fluor 350 conjugate   Wheat germ agglutinin, Alexa Fluor 488 conjugate   Wheat germ agglutinin, Alexa Fluor 555 conjugate Wheat germ agglutinin, Alexa Fluor 594 conjugate Wheat germ agglutinin, Alexa Fluor 647 conjugate  
Blue Cyan Green Orange Red Deep Red

Cell segmentation

Plasma membrane segmentation
    CellMask Green plasma membrane stain CellMask Orange plasma membrane stain   CellMask Deep Red plasma membrane stain
Nuclear segmentation
HCS NuclearMask Blue   SYTO 9 Green fluorescent nucleic acid stain SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red

Cell tracking & tracing

CellTracker Blue CellTracker Violet CellTracker Green CellTracker Orange CellTracker Red CellTracker Deep Red

Cell structure

Cytoskeleton—tubulin
    Tubulin Tracker Green      
Endoplasmic reticulum
    ER-Tracker Green (BODIPY FL Glibenclamide)   ER-Tracker Red (BODIPY TR Glibenclamide)  
Lysosomes
    LysoTracker Green   LysoTracker Red DND-99 LysoTracker Deep Red
Mitochondria
    MitoTracker Green FM MitoTracker Orange CMTMRos MitoTracker Red CM-H2XRos MitoTracker Deep Red FM

Cell function probes

Cell viability
    Image-iT DEAD Green viability stain   Propidium iodide  
Cell proliferation
    Click-iT EdU Alexa Fluor 488 HCS Assay Click-iT EdU Alexa Fluor 555 HCS Assay Click-iT EdU Alexa Fluor 594 HCS Assay Click-iT EdU Alexa Fluor 647 HCS Assay
Apoptosis—caspase activity
    CellEvent Caspase-3/7 Green Detection Reagent      
Apoptosis—DNA fragmentation TUNEL
    Click-iT TUNEL Alexa Fluor 488 Imaging Assay   Click-iT TUNEL Alexa Fluor 594 Imaging Assay Click-iT TUNEL Alexa Fluor 647 Imaging Assay
Oxidative stress—general
    CellROX Green reagent     CellROX Deep Red reagent
Endocytosis—lysosome-directed
    pHrodo Green Dextran, 10,000 MW for endocytosis pHrodo Red Dextran, 10,000 MW for endocytosis    
    Dextran, Alexa Fluor 488, 10,000 MW Dextran, Alexa Fluor 555, 10,000 MW Dextran, Alexa Fluor 594, 10,000 MW Dextran, Alexa Fluor 647, 10,000 MW
Endocytosis—receptor-mediated
    Transferrin from human serum, Alexa Fluor 488 conjugate     Transferrin from human serum, Alexa Fluor 647 conjugate
Cell cycle
    Premo FUCCI Cell Cycle Sensor Premo FUCCI Cell Cycle Sensor    
Autophagy
    Premo Autophagy Tandem Sensor RFP-GFP-LC3B Premo Autophagy Tandem Sensor RFP-GFP-LC3B    

New protein synthesis

Click-iT HPG
    Alexa Fluor 488 Azide Alexa Fluor 555 Azide Alexa Fluor 594 Azide Alexa Fluor 647 Azide
Click-iT AHA
    Alexa Fluor 488 Alkyne Alexa Fluor 555 Alkyne Alexa Fluor 594 Alkyne Alexa Fluor 647 Alkyne
Click-iT OPP
    Alexa Fluor 488 Picolyl Azide Toolkit Alexa Fluor 555 Picolyl Azide Toolkit   Alexa Fluor 647 Picolyl Azide Toolkit
Blue Cyan Green/Yellow Orange Red Deep Red Near IR

Antibody labels for secondary detection

Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 750
    Goat anti-chicken IgY   Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY  
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
Goat anti-rat IgG   Goat anti-rat IgG   Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG  
    Goat anti-guinea pig IgG   Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG  
    Goat anti-human IgG   Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG  
Donkey anti-goat IgG   Donkey anti-goat IgG   Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG  
    Donkey anti-mouse IgG   Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG  
    Donkey anti-rat IgG     Donkey anti-rat IgG    
    Rabbit anti-goat IgG   Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG  
Rabbit anti-mouse IgG   Rabbit anti-mouse IgG   Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG  
    Rabbit anti-rat IgG     Rabbit anti-rat IgG    
    Chicken anti-goat IgG     Chicken anti-goat IgG Chicken anti-goat IgG  
    Chicken anti-mouse IgG     Chicken anti-mouse IgG Chicken anti-mouse IgG  
    Chicken anti-rabbit IgG     Chicken anti-rabbit IgG Chicken anti-rabbit IgG  
    Chicken anti-rat IgG     Chicken anti-rat IgG Chicken anti-rat IgG  

Cell segmentation

Whole cell segmentation
HCS CellMask Blue   HCS CellMask Green   HCS CellMask Orange   HCS CellMask Deep Red  
Nuclear segmentation
HCS NuclearMask  Blue   SYTO 9 Green fluorescent nucleic acid stain   SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red  
DAPI   SYTOX Green nucleic acid stain     HCS NuclearMask Red TO-PRO-3 stain  

Cell structure

Cytoskeleton—actin
Alexa Fluor 350 phalloidin   Alexa Fluor 488 phalloidin   Alexa Fluor 555 phalloidin Alexa Fluor 594 phalloidin Alexa Fluor 647 phalloidin  
Plasma membrane
Wheat germ agglutinin, Alexa Fluor 350 conjugate   Wheat germ agglutinin, Alexa Fluor 488 conjugate   Wheat germ agglutinin, Alexa Fluor 555 conjugate Wheat germ agglutinin, Alexa Fluor 594 conjugate Wheat germ agglutinin, Alexa Fluor 647 conjugate  
Blue Cyan Green Orange Red Deep Red

Cell segmentation

Plasma membrane segmentation
    CellMask Green plasma membrane stain CellMask Orange plasma membrane stain   CellMask Deep Red plasma membrane stain
Nuclear segmentation
HCS NuclearMask Blue   SYTO 9 Green fluorescent nucleic acid stain SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red

Cell tracking & tracing

CellTracker Blue CellTracker Violet CellTracker Green CellTracker Orange CellTracker Red CellTracker Deep Red

Cell structure

Cytoskeleton—tubulin
    Tubulin Tracker Green      
Endoplasmic reticulum
    ER-Tracker Green (BODIPY FL Glibenclamide)   ER-Tracker Red (BODIPY TR Glibenclamide)  
Lysosomes
    LysoTracker Green   LysoTracker Red DND-99 LysoTracker Deep Red
Mitochondria
    MitoTracker Green FM MitoTracker Orange CMTMRos MitoTracker Red CM-H2XRos MitoTracker Deep Red FM

Cell function probes

Cell viability
    Image-iT DEAD Green viability stain   Propidium iodide  
Cell proliferation
    Click-iT EdU Alexa Fluor 488 HCS Assay Click-iT EdU Alexa Fluor 555 HCS Assay Click-iT EdU Alexa Fluor 594 HCS Assay Click-iT EdU Alexa Fluor 647 HCS Assay
Apoptosis—caspase activity
    CellEvent Caspase-3/7 Green Detection Reagent      
Apoptosis—DNA fragmentation TUNEL
    Click-iT TUNEL Alexa Fluor 488 Imaging Assay   Click-iT TUNEL Alexa Fluor 594 Imaging Assay Click-iT TUNEL Alexa Fluor 647 Imaging Assay
Oxidative stress—general
    CellROX Green reagent     CellROX Deep Red reagent
Endocytosis—lysosome-directed
    pHrodo Green Dextran, 10,000 MW for endocytosis pHrodo Red Dextran, 10,000 MW for endocytosis    
    Dextran, Alexa Fluor 488, 10,000 MW Dextran, Alexa Fluor 555, 10,000 MW Dextran, Alexa Fluor 594, 10,000 MW Dextran, Alexa Fluor 647, 10,000 MW
Endocytosis—receptor-mediated
    Transferrin from human serum, Alexa Fluor 488 conjugate     Transferrin from human serum, Alexa Fluor 647 conjugate
Cell cycle
    Premo FUCCI Cell Cycle Sensor Premo FUCCI Cell Cycle Sensor    
Autophagy
    Premo Autophagy Tandem Sensor RFP-GFP-LC3B Premo Autophagy Tandem Sensor RFP-GFP-LC3B    

New protein synthesis

Click-iT HPG
    Alexa Fluor 488 Azide Alexa Fluor 555 Azide Alexa Fluor 594 Azide Alexa Fluor 647 Azide
Click-iT AHA
    Alexa Fluor 488 Alkyne Alexa Fluor 555 Alkyne Alexa Fluor 594 Alkyne Alexa Fluor 647 Alkyne
Click-iT OPP
    Alexa Fluor 488 Picolyl Azide Toolkit Alexa Fluor 555 Picolyl Azide Toolkit   Alexa Fluor 647 Picolyl Azide Toolkit