Plasmid DNA Maxiprep Kits

A plasmid maxiprep is a laboratory technique used to isolate and purify a large amount of plasmid DNA from bacterial cultures on a large scale. It is a step up from the smaller scale miniprep and midiprep methods. The maxiprep procedure typically involves similar steps to the midiprep, such as bacterial cell lysis, removal of cellular debris, and purification of the plasmid DNA. However, the maxiprep yields a significantly higher quantity of plasmid DNA, usually in the range of several hundred micrograms to milligrams, making it suitable for applications that require a large amount of pure plasmid DNA, such as large-scale cloning, protein production, or viral vector production.

A maxiprep scale kit should be used when attempting to purify plasmid from an overnight bacterial culture with a volume between 100–500 mL. When choosing a plasmid maxiprep kit, scientists should prioritize high-quality plasmid DNA that is pure, intact, and free from contaminants. This is crucial for helping ensure reliable and reproducible results in downstream applications, helping instill confidence in the experimental outcomes.

The KingFisher PlasmidPro Maxi Processor is designed to help simplify the maxi scale plasmid purification process—from sample loading to elution. Simply pour in your culture and press start. In under 75 minutes, this innovative system efficiently executes all important purification steps to deliver endotoxin-free, high-quality plasmid DNA without manual steps or centrifugation.

The Thermo Fisher Scientific GeneJET Plasmid Maxiprep Kit allows for efficient isolation of high-quality plasmid DNA from bacterial cultures, recovering up to 400 μg per prep. With a fast 60-minute procedure and a convenient spin column format, this kit offers high yields and concentrations, making it suitable for various molecular biology procedures.

The PureLink HiPure Plasmid Maxiprep Kit isolates transfection-grade plasmid DNA from E. coli, yielding up to 1 mg of highly pure DNA. With no extra steps required to remove contaminants, this kit can help save time and labor. It is versatile, suitable for purifying various types and sizes of plasmid DNA, including BAC, bacmids, and ssM13 DNAs.

Use the table below to help you choose the right solution for your plasmid DNA maxiprep.

Products sorted by Endotoxin level from lowest EU/µg to highest associated with purity grade.

Explore: Plasmid DNA Miniprep
Explore: Plasmid DNA Midiprep