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Two-color nuclear staining assay for cell viability

This kit can be used to quickly and easily determine the viability of cells. NucBlue Live reagent stains the nuclei of all cells, while NucGreen Dead reagent stains only the nuclei of dead cells.

This protocol can be used for:

  • Identifying live and dead cells using a fluorescence microscope

This protocol should not be used for:

  • Flow cytometry

You will need the following for this protocol:

Protocol

1. Culture cells in appropriate medium and vessel for microscopy
2. Add 2 drops each NucBlue Live and NucGreen Dead reagents per milliliter of medium to label cells
3. Incubate 5–30 minutes
4. Image cells

Spectral information and storage

  NucBlue Live NucGreen Dead
Excitation/Emission 360/460 nm 504/523 nm
Standard filter set DAPI FITC/GFP
EVOS Light Cube DAPI GFP
Storage conditions Room temperature Room temperature

 

Protocol tips

Image of cells stained with NucBlue Live and NucGreen Dead reagents
Jurkat cells stained with ReadyProbes (Blue/Green) Cell Viability Imaging Kit.