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Invitrogen
Mouse PD-L1 (CD274) ELISA Kit
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Product Specifications
Analytical sensitivity
0.06 ng/mL
Assay range
0.055-15 ng/mL
Sample type/volume
Supernatant
100 µL
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
PD-L1 (CD274)
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse PD-L1 (CD274) ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of mouse PD-L1/B7-H1 in serum, plasma, and cell culture supernatants.
Target information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : A530045L16Rik, B7h1, Cd274, Pdcd1l1, Pdcd1lg1, Pdl1
Gene ID : (Mouse) 60533
Gene symbol : Cd274
Protein Aliases : B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, Programmed cell death 1 ligand 1, programmed death ligand 1, sCD274, soluble CD274, soluble PD L1, sPD L1, pdl-1
UniProt ID (Mouse) Q9EP73
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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