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Reverse transcriptases (RTs) synthesize complementary DNA (cDNA) from RNA templates for applications including RT-qPCR, RT-PCR, RNA sequencing, and gene expression analysis. Choose from standalone reverse transcriptase enzymes, master mixes, and cDNA synthesis kits designed for routine, challenging, low-input, and high-throughput workflows.
Choose the right cDNA synthesis solution for your application
SuperScript V reverse transcription products
Invitrogen SuperScript V product family is the next generation novel solution for cDNA synthesis, offering excellent performance, easy reaction set up, and exceptional stability. SuperScript V products designed to offer excellent sensitivity and robust performance across a wide range of samples, including GC-rich, structured, degraded, and inhibitor-rich RNA. It also features workflow innovations, including rapid reaction times, benchtop stability of assembled reactions, and flexible priming options which allow greater versatility and control at the bench. In addition, its stability features support room temperature shipping, storage, and set up.
Quick tips to choose
- Need maximum flexibility? Choose SuperScript V Reverse Transcriptase
- Want the simplest routine two-step RT-qPCR setup? Choose SuperScript V VILO Master Mix
- Want master-mix convenience but need to choose your own primers? Choose SuperScript V VILO Master Mix, Primer-Free
Specialty cDNA synthesis kits
Beyond our core reverse transcriptase and master mix formats, the SuperScript portfolio includes specialized kits optimized for specific cDNA synthesis workflows. These solutions extend SuperScript performance to applications such as one-step RT-PCR, low-input preamplification, template switching, and direct cDNA synthesis.
These kits enable simplified workflow, fewer pipetting steps that lower risk of errors and contamination, and suitability for high-throughput applications.
Additional products
Additional reverse transcription solutions are available for specialized, routine, and value-focused workflows.
FAQs
Choose a standalone reverse transcriptase when you want maximum flexibility to select primers and optimize reaction conditions.
Choose a master mix when you want a streamlined workflow with fewer setup steps; options are available with optimized primers or without primers for greater priming flexibility.
Choose a kit when you want an optimized solution for simplified or high-throughput workflows.
Consider a high-performance reverse transcriptase such as SuperScript V Reverse Transcriptase designed to work across challenging sample types including GC-rich, structured, degraded, and inhibitor-rich RNA and can detect as low as 100 fg input RNA.
For very low-input or single-cell samples, consider solutions specifically designed for preamplification.
For routine two-step RT-qPCR, a ready-to-use master mix can simplify reaction setup. SuperScript V VILO Master Mix contains optimized oligo (dT) and random primers, while the primer-free format provides the convenience of a master mix with flexibility to choose the priming strategy.
Choose a one-step RT-PCR kit when you want reverse transcription and amplification to occur in a single workflow.
Direct reverse transcription products are designed to eliminate a separate RNA-isolation step by combining cell lysis and cDNA synthesis in a single tube.
Our value brand products are manufactured to the highest quality standards, widely viewed as premium brands and well cited in publications.
| Premium performance: SuperScript V products | Enhanced value: Maxima H Minus products | Routine economy: High Capacity products |
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Related products
In addition to a vast collection of RT reagents, we offer several products to support your entire workflow.
Resources
For Research Use Only. Not for use in diagnostic procedures.